...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Genetic Linkage and Cotransfer of a Novel,vanB-Containing Transposon (Tn5382) and a Low-Affinity Penicillin-Binding Protein 5 Gene in a Clinical Vancomycin-Resistant Enterococcus faecium Isolate
【24h】

Genetic Linkage and Cotransfer of a Novel,vanB-Containing Transposon (Tn5382) and a Low-Affinity Penicillin-Binding Protein 5 Gene in a Clinical Vancomycin-Resistant Enterococcus faecium Isolate

机译：新型Van含转座子（Tn5382）和低亲和力青霉素结合蛋白5基因在临床抗万古霉素肠球菌粪便分离物中的遗传连锁和共转移

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mechanisms for the intercellular transfer of VanB-type vancomycin resistance determinants and for the almost universal association of these determinants with those for high-level ampicillin resistance remain poorly defined. We report the discovery of Tn5382, a ca. 27-kb putative transposon encoding VanB-type glycopeptide resistance in Enterococcus faecium. Open reading frames internal to the right end of Tn5382 and downstream of thevanX B dipeptidase gene exhibit significant homology to genes encoding the excisase and integrase of conjugative transposon Tn916. The ends of Tn5382 are also homologous to the ends of Tn916, especially in regions bound by the integrase enzyme. PCR amplification experiments indicate that Tn5382 excises to form a circular intermediate inE. faecium. Integration of Tn5382 in the chromosome of E. faecium C68 has occurred 113 bp downstream of the stop codon for the pbp5 gene, which encodes high-level ampicillin resistance in this clinical isolate. Transfer of vancomycin, ampicillin, and tetracycline resistance from C68 to anE. faecium recipient strain occurs at low frequency in vitro and is associated with acquisition of a 130- to 160-kb segment of DNA that contains Tn5382, the pbp5 gene, and its putative repressor gene, psr. The interenterococcal transfer of this large chromosomal element appears to be the primary mechanism for vanB operon spread in northeast Ohio. These results expand the known family of Tn916-related transposons, suggest a mechanism for vanB operon entry into and dissemination among enterococci, and provide an explanation for the nearly universal association of vancomycin and high-level ampicillin resistance in clinical E. faecium strains.
机译：尚不清楚VanB型万古霉素耐药性决定簇在细胞间转移的机制以及这些决定簇与高水平氨苄青霉素耐药性几乎普遍相关的机制。我们报告了Tn 5382 的发现。粪肠球菌中编码VanB型糖肽耐药性的27kb假定转座子。 Tn 5382 右端内部和 vanX B 二肽酶基因下游的开放阅读框与编码酶和整合酶的基因具有显着同源性接合转座子Tn 916 Tn 5382 的末端也与Tn 916 的末端同源，特别是在整合酶结合的区域。 PCR扩增实验表明，Tn 5382 切除后在 E中形成环状中间体。粪便。 Tn 5382 在 E染色体中的整合。粪 C68出现在 pbp5 基因终止密码子下游113 bp处，该基因在此临床分离株中编码高水平的氨苄青霉素抗性。万古霉素，氨苄青霉素和四环素耐药性从C68转移至anemE。粪便受体菌株在体外低频率发生，并与含有Tn 5382 ， pbp5 的130-160kb DNA片段的获取有关基因及其推定的阻遏物基因 psr 。这种大染色体元件的肠球菌间转移似乎是 vanB 操纵子在俄亥俄州东北部扩散的主要机制。这些结果扩展了已知的Tn 916 相关转座子家族，为 vanB 操纵子进入肠球菌和在肠球菌中传播提供了一种机制，并为近乎普遍的肠球菌协会提供了解释。万古霉素和高水平的氨苄青霉素耐药性在临床上。屎菌

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号