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首页> 外文期刊>Journal of bacteriology >Chromate Efflux by Means of the ChrA Chromate Resistance Protein from Pseudomonas aeruginosa
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Chromate Efflux by Means of the ChrA Chromate Resistance Protein from Pseudomonas aeruginosa

机译:通过铜绿假单胞菌的ChrA铬酸盐抗性蛋白对铬酸盐的流出

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Everted membrane vesicles of Pseudomonas aeruginosaPAO1 harboring plasmid pCRO616, expressing the ChrA chromate resistance protein, accumulated four times more51CrO4 2? than vesicles from plasmidless cells, indicating that a chromate efflux system functions in the resistant strain. Chromate uptake showed saturation kinetics with an apparent Km of 0.12 mM chromate and aV max of 0.5 nmol of chromate/min per mg of protein. Uptake of chromate by vesicles was dependent on NADH oxidation and was abolished by energy inhibitors and by the chromate analog sulfate. The mechanism of resistance to chromate determined by ChrA appears to be based on the active efflux of chromate driven by the membrane potential.
机译:带有质粒pCRO616的铜绿假单胞菌PAO1的外翻膜小泡表达ChrA铬酸抗性蛋白,其积累量是 51 CrO 4 2比无质粒细胞的囊泡更强,表明铬酸盐外排系统在抗性菌株中起作用。铬酸盐的吸收表现出饱和动力学,表观 K m 为0.12 mM铬酸盐,a V max 为0.5 nmol。每毫克蛋白质每分钟的铬酸盐含量。囊泡对铬酸盐的吸收取决于NADH的氧化,并被能量抑制剂和铬酸盐类似物硫酸盐废除。由ChrA确定的对铬酸盐的抗性机制似乎是基于膜电位驱动的铬酸盐的主动流出。

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