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首页> 外文期刊>Journal of bacteriology >Identification and Characterization of Major Lipid Particle Proteins of the Yeast Saccharomyces cerevisiae
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Identification and Characterization of Major Lipid Particle Proteins of the Yeast Saccharomyces cerevisiae

机译：酵母酵母主要脂质颗粒蛋白的鉴定与表征

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Lipid particles of the yeast Saccharomyces cerevisiaewere isolated at high purity, and their proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Major lipid particle proteins were identified by mass spectrometric analysis, and the corresponding open reading frames (ORFs) were deduced. In silicio analysis revealed that all lipid particle proteins contain several hydrophobic domains but none or only few (hypothetical) transmembrane spanning regions. All lipid particle proteins identified by function so far, such as Erg1p, Erg6p, and Erg7p (ergosterol biosynthesis) and Faa1p, Faa4p, and Fat1p (fatty acid metabolism), are involved in lipid metabolism. Based on sequence homology, another group of three lipid particle proteins may be involved in lipid degradation. To examine whether lipid particle proteins of unknown function are also involved in lipid synthesis, mutants with deletions of the respective ORFs were constructed and subjected to systematic lipid analysis. Deletion of YDL193w resulted in a lethal phenotype which could not be suppressed by supplementation with ergosterol or fatty acids. Other deletion mutants were viable under standard conditions. Strains with YBR177c, YMR313c, and YKL140w deleted exhibited phospholipid and/or neutral lipid patterns that were different from the wild-type strain and thus may be further candidate ORFs involved in yeast lipid metabolism.
机译：以高纯度分离出酿酒酵母酵母的脂质颗粒，并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离蛋白质。通过质谱分析鉴定了主要的脂质颗粒蛋白，并推导了相应的开放阅读框（ORF）。在硅质分析中显示，所有脂质颗粒蛋白都包含几个疏水域，但没有或只有很少（假设的）跨膜区域。到目前为止，通过功能鉴定的所有脂质颗粒蛋白，例如Erg1p，Erg6p和Erg7p（麦角固醇生物合成）以及Faa1p，Faa4p和Fat1p（脂肪酸代谢）都参与脂质代谢。基于序列同源性，另一组三种脂质颗粒蛋白可能参与脂质降解。为了检查未知功能的脂质颗粒蛋白是否也参与脂质合成，构建了具有各自ORF缺失的突变体，并进行了系统的脂质分析。 YDL193w的缺失导致致死表型，其不能通过补充麦角固醇或脂肪酸来抑制。其他缺失突变体在标准条件下是可行的。缺失YBR177c，YMR313c和YKL140w的菌株表现出与野生型菌株不同的磷脂和/或中性脂质模式，因此可能是参与酵母脂质代谢的其他候选ORF。

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