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首页> 外文期刊>Journal of bacteriology >The Yersinia enterocoliticaPhospholipase Gene yplA Is Part of the Flagellar Regulon
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The Yersinia enterocoliticaPhospholipase Gene yplA Is Part of the Flagellar Regulon

机译:小肠结肠炎耶尔森氏菌磷脂酶基因yplA是鞭毛调节基因的一部分

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摘要

Yersinia enterocolitica yplA encodes a phospholipase required for virulence. Virulence genes are often regulated in response to environmental signals; therefore, yplA expression was examined using a yplA::lacZYtranscriptional fusion. Maximal yplA expression occurred between pH 6.5 and pH 7.5 and was induced in the mid-logarithmic growth phase. Potential Fnr, cyclic AMP (cAMP)-cAMP receptor protein (Crp), and ?F regulatory sites were identified in the nucleotide sequence. Reduction of yplA expression by aeration, addition of glucose and sucrose, and application of high temperature and salt is consistent with Fnr-, cAMP-Crp-, and ?F-mediated regulation, respectively. Expression ofyplA was reduced in flhDC and fliAnull strains, indicating that yplA is part of the flagellar regulon.
机译:小肠结肠炎耶尔森氏菌yplA 编码一种毒性所需的磷脂酶。毒力基因通常根据环境信号进行调控。因此,使用 yplA :: lacZY 转录融合技术检查了 yplA 表达。最大的 yplA 表达发生在pH 6.5和pH 7.5之间,并在对数生长期中期诱导。在核苷酸序列中鉴定出潜在的Fnr,环状AMP(cAMP)-cAMP受体蛋白(Crp)和α F 调控位点。通过通气,添加葡萄糖和蔗糖以及施加高温和盐降低 yplA 表达,与Fnr-,cAMP-Crp-和? F 介导的一致监管。在 flhDC fliA null株中, yplA 的表达降低,表明 yplA 是鞭毛调节子的一部分。

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