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首页> 外文期刊>Journal of bacteriology >A ?54 Activator Protein Necessary for Spore Differentiation within the Fruiting Body ofMyxococcus xanthus
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A ?54 Activator Protein Necessary for Spore Differentiation within the Fruiting Body ofMyxococcus xanthus

机译：粘果球菌子实体内孢子分化所必需的？54活化蛋白

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Insertion of an internal DNA fragment into the act1gene, which encodes one of several ?54-activator proteins in Myxococcus xanthus, produced a mutant defective in fruiting body development. While fruiting-body aggregation appears normal in the mutant, it fails to sporulate (<10?6 the wild-type number of viable spores). The A and C intercellular signals, which are required for sporulation, are produced by the mutant. But, while it produces A-factor at levels as high as that of the wild type, the mutant produces much less C-signal than normal, as measured either by C-factor bioassay or by the total amount of C-factor protein detected with specific antibody. Expression of three C-factor-dependent reporters is altered in the mutant: the level of expression of Ω4414 is about 15% of normal, and Ω4459 and Ω4403 have alterations in their time course. Finally, the methylation of FrzCD protein is below normal in the mutant. It is proposed that Act1 protein responds to C-signal reception by increasing the expression of the csgAgene. This C-signal-dependent increase constitutes a positive feedback in the wild type. The act1 mutant, unable to raise the level of csgA expression, carries out only those developmental steps for which a low level of C-signaling is adequate.
机译：将内部DNA片段插入 act1 基因中，该基因编码 Myxococcus xanthus 中的几种？ 54 -激活蛋白之一。在子实体发育中。虽然突变体中的子实体聚集正常，但不能形成孢子（<10 ？6 活孢子的野生型数量）。孢子形成所必需的A和C细胞间信号是由突变体产生的。但是，尽管它产生的A因子水平与野生型一样高，但突变体产生的C信号比正常情况下少得多，无论是通过C因子生物测定还是通过C因子检测到的C因子蛋白总量进行测量特异性抗体。突变体中三个C因子依赖性报告基因的表达发生了变化：Ω4414的表达水平约为正常水平的15％，而Ω4459和Ω4403的表达随时间变化。最后，在突变体中，FrzCD蛋白的甲基化低于正常水平。有人提出Act1蛋白通过增加 csgA 基因的表达来响应C信号的接收。这种依赖C信号的增加在野生型中构成正反馈。 act1 突变体无法提高 csgA 表达水平，仅执行低C信号水平就足够的发育步骤。

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