Cloning and Sequence Analysis of the Mercury Resistance Operon of Streptomyces sp. Strain CHR28 Reveals a Novel Putative Second Regulatory Gene

Jacques Ravel; Jocelyne DiRuggiero; Frank T. Robb; Russell T. Hill

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Cloning and Sequence Analysis of the Mercury Resistance Operon of Streptomyces sp. Strain CHR28 Reveals a Novel Putative Second Regulatory Gene

机译：链霉菌水银抗性操纵子的克隆与序列分析。菌株CHR28揭示了一个新的推定的第二调节基因。

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A DNA library of pRJ28, a large linear plasmid encoding mercury resistance, was constructed, and the mercury resistance genes were cloned. The 5,921-bp sequence was analyzed and showed a high degree of similarity to the Streptomyces lividans 1326 mercury resistance operon. Genes merR, merT,merP, and orfIV were found in a similar order and in a single transcription unit. merA andmerB were found to be transcribed in the opposite direction to genes merR, merT, merP, andorfIV, as in S. lividans 1326. A novel putative regulatory gene, orfX, was found 22 bp downstream ofmerA. orfX encodes a 137-amino acid protein with a potential helix-turn-helix motif in the N-terminal domain, characteristic of the MerR family of transcriptional regulators. Transcriptional studies showed that orfX is cotranscribed with merA and merB. It is hypothesized thatorfX plays a role in the regulation of the mercury resistance operon, probably by binding at the MerR operator site.

机译：构建了pRJ28的DNA文库，pRJ28是编码耐汞性的大型线性质粒，并克隆了耐汞基因。分析了5,921-bp的序列，并显示出与 Streptomyces lividans 1326耐汞操纵子的高度相似性。发现基因 merR ， merT ， merP 和 orfIV 的顺序相似且位于单个转录单位中。发现 merA 和 merB 的转录方向与 merR ， merT ， merP基因相反和 orfIV ，如 S。 lividans 1326.在 merA下游22 bp处发现了一个新的推定调控基因 orfX 。 orfX 编码一个137个氨基酸的蛋白质，在N末端域具有潜在的螺旋-转-螺旋基序，这是转录调节子MerR家族的特征。转录研究表明， orfX 与 merA 和 merB 共转录。假设 orfX 可能在MerR操纵子位点结合，从而在耐汞操纵子的调节中发挥作用。 展开▼

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《Journal of bacteriology》 |2000年第8期|共5页

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Jacques Ravel; Jocelyne DiRuggiero; Frank T. Robb; Russell T. Hill;
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6. Note: Cloning and Sequence Analysis of the Mercury Resistance Operon of Streptomyces sp. Strain CHR28 Reveals a Novel Putative Second Regulatory Gene [O] . Jacques Ravel, Jocelyne DiRuggiero, Frank T. Robb, 2000

机译：注意：链霉菌的耐汞操纵子的克隆和序列分析。菌株CHR28揭示了一个新的推定的第二调节基因。

7. Cloning and Sequence Analysis of the Mercury Resistance Operon of Streptomyces sp. Strain CHR28 Reveals a Novel Putative Second Regulatory Gene [O] . Jacques Ravel, Jocelyne DiRuggiero, Frank T. Robb, 2000

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Cloning and Sequence Analysis of the Mercury Resistance Operon of Streptomyces sp. Strain CHR28 Reveals a Novel Putative Second Regulatory Gene

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