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首页> 外文期刊>Journal of bacteriology >Role of Ger Proteins in Nutrient and Nonnutrient Triggering of Spore Germination in Bacillus subtilis
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Role of Ger Proteins in Nutrient and Nonnutrient Triggering of Spore Germination in Bacillus subtilis

机译：Ger蛋白在枯草芽孢杆菌孢子萌发中的营养和非营养触发

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Dormant Bacillus subtilis spores germinate in the presence of particular nutrients called germinants. The spores are thought to recognize germinants through receptor proteins encoded by the gerA family of operons, which includesgerA, gerB, and gerK. We sought to substantiate this putative function of the GerA family proteins by characterizing spore germination in a mutant strain that contained deletions at all known gerA-like loci. As expected, the mutant spores germinated very poorly in a variety of rich media. In contrast, they germinated like wild-type spores in a chemical germinant, a 1-1 chelate of Ca2+ and dipicolinic acid (DPA). These observations showed that proteins encoded bygerA family members are required for nutrient-induced germination but not for chemical-triggered germination, supporting the hypothesis that the GerA family encodes receptors for nutrient germinants. Further characterization of Ca2+–DPA-induced germination showed that the effect of Ca2+–DPA on spore germination was saturated at 60 mM and had a Km of 30 mM. We also found that decoating spores abolished their ability to germinate in Ca2+–DPA but not in nutrient germinants, indicating that Ca2+–DPA and nutrient germinants probably act through parallel arms of the germination pathway.
机译：枯草芽孢杆菌的孢子在称为萌发剂的特定营养素存在下发芽。人们认为孢子通过 gerA 操纵子家族编码的受体蛋白来识别发芽细菌，这些受体蛋白包括 gerA ， gerB 和 gerK 。我们试图通过表征突变株中芽孢的萌发来证实GerA家族蛋白的这种推定功能，该突变株在所有已知的 gerA 样位点均具有缺失。不出所料，突变孢子在各种富营养培养基中的发芽非常差。相反，它们在化学发芽剂，Ca 2 + 和二吡啶甲酸（DPA）的1-1螯合物中像野生型孢子一样发芽。这些观察结果表明， gerA 家族成员编码的蛋白质对于营养诱导的萌发是必需的，而对于化学触发的萌发则不是必需的，这支持了GerA家族编码营养发芽剂受体的假设。 Ca 2 + –DPA诱导的发芽的进一步表征表明，Ca 2 + –DPA对孢子发芽的作用在60 mM时达到饱和并且具有 K m 为30 mM。我们还发现脱壳的孢子消除了它们在Ca 2 + -DPA中发芽的能力，但在营养发芽剂中却没有，表明Ca 2 + -DPA和营养发芽剂可能通过发芽途径的平行臂。

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