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首页> 外文期刊>Journal of bacteriology >Deletion of the min Operon Results in Increased Thermosensitivity of an ftsZ84 Mutant and Abnormal FtsZ Ring Assembly, Placement, and Disassembly
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Deletion of the min Operon Results in Increased Thermosensitivity of an ftsZ84 Mutant and Abnormal FtsZ Ring Assembly, Placement, and Disassembly

机译：删除最小操纵子会导致ftsZ84突变体的热敏性增加，并且FtsZ环组装，放置和拆卸异常。

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To investigate the interaction between FtsZ and the Min system during cell division of Escherichia coli, we examined the effects of combining a well-known thermosensitive mutation offtsZ, ftsZ84, with ΔminCDE, a deletion of the entire min locus. Because the Min system is thought to down-regulate Z-ring assembly, the prediction was that removing minCDE might at least partially suppress the thermosensitivity of ftsZ84, which can form colonies below 42°C but not at or above 42°C. Contrary to expectations, the double mutant was significantly more thermosensitive than theftsZ84 single mutant. When shifted to the new lower nonpermissive temperature, the double mutant formed long filaments mostly devoid of Z rings, suggesting a likely cause of the increased thermosensitivity. Interestingly, even at 22°C, many Z rings were missing in the double mutant, and the rings that were present were predominantly at the cell poles. Of these, a large number were present only at one pole. These cells exhibited a higher than expected incidence of polar divisions, with a bias toward the newest pole. Moreover, some cells exhibited dramatically elongated septa that stained for FtsZ, suggesting that the double mutant is defective in Z-ring disassembly, and providing a possible mechanism for the polar bias. Thermoresistant suppressors of the double mutant arose that had modestly increased levels of FtsZ84. These cells also exhibited elongated septa and, in addition, produced a high frequency of branched cells. A thermoresistant suppressor of the ftsZ84 single mutant also synthesized more FtsZ84 and produced branched cells. The evidence from this study indicates that removing the Min system exposes and exacerbates the inherent defects of the FtsZ84 protein, resulting in clear septation phenotypes even at low growth temperatures. Increasing levels of FtsZ84 can suppress some, but not all, of these phenotypes.
机译：为了研究大肠杆菌细胞分裂过程中FtsZ和Min系统之间的相互作用，我们研究了结合 ftsZ ， ftsZ84的众所周知的热敏突变的作用，带有Δ minCDE ，则删除了整个 min 位点。由于人们认为Min系统会下调Z环的装配，因此预测是去除 minCDE 可能会至少部分抑制 ftsZ84 的热敏性，并可能在下面形成菌落。 42°C，但不高于或等于42°C。与预期相反，双突变体比 ftsZ84 单突变体对热敏感。当转移到新的较低的不允许的温度时，双突变体形成了长丝，大部分没有Z环，表明可能是引起热敏性增加的原因。有趣的是，即使在22°C下，双突变体中也缺少许多Z环，并且存在的环主要位于细胞极。其中，大量仅存在于一个极点。这些细胞表现出高于预期的极性分裂发生率，并且偏向最新的极点。此外，一些细胞表现出显着延长的间隔，该间隔被FtsZ染色，表明双突变体在Z环拆卸方面存在缺陷，并为极性偏倚提供了可能的机制。出现了双突变体的耐热抑制剂，其FtsZ84的水平适度增加。这些细胞还表现出拉长的隔片，此外，还产生高频率的分支细胞。 ftsZ84 单个突变体的耐热抑制剂也合成了更多的FtsZ84，并产生了分支细胞。这项研究的证据表明，去除Min系统会暴露并加剧FtsZ84蛋白的固有缺陷，即使在较低的生长温度下，也会产生清晰的分离表型。 FtsZ84水平的提高可以抑制其中的一些，但不是全部。

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