VpsR, a Member of the Response Regulators of the Two-Component Regulatory Systems, Is Required for Expression ofvps Biosynthesis Genes and EPSETr-Associated Phenotypes in Vibrio cholerae O1 El Tor

Fitnat H. Yildiz; Nadia A. Dolganov; Gary K. Schoolnik

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VpsR, a Member of the Response Regulators of the Two-Component Regulatory Systems, Is Required for Expression ofvps Biosynthesis Genes and EPSETr-Associated Phenotypes in Vibrio cholerae O1 El Tor

机译：VpsR是两组分调节系统响应调节器的成员，对于霍乱弧菌O1 El Tor中vps生物合成基因和EPSETr相关表型的表达是必需的

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The rugose colonial variant of Vibrio cholerae O1 El Tor produces an exopolysaccharide (EPSETr) that enables the organism to form a biofilm and to resist oxidative stress and the bactericidal action of chlorine. Transposon mutagenesis of the rugose variant led to the identification of vpsR, which codes for a homologue of the NtrC subclass of response regulators. Targeted disruption of vpsR in the rugose colony genetic background yielded a nonreverting smooth-colony morphotype that produced no detectable EPSETr and did not form an architecturally mature biofilm. Analysis of two genes, vpsA andvpsL, within the vps cluster of EPSETr biosynthesis genes revealed that their expression is induced above basal levels in the rugose variant, compared to the smooth colonial variant, and requires vpsR. These results show that VpsR functions as a positive regulator of vpsAand vpsL and thus acts to positively regulate EPSETr production and biofilm formation.

机译：霍乱弧菌 O1 El Tor的皱纹菌落产生一种胞外多糖（EPS ETr ），使生物能够形成生物膜并抵抗氧化应激和氯的杀菌作用。皱纹变体的转座子诱变导致 vpsR 的鉴定，该序列编码应答调节子的NtrC亚类的同源物。在皱纹菌落的遗传背景中对 vpsR 的靶向破坏产生了不可逆的平滑菌落形态，没有产生可检测的EPS ETr ，并且没有形成结构上成熟的生物膜。在EPS ETr 生物合成基因的 vps 簇中的两个基因 vpsA 和 vpsL 的分析表明，它们的表达与平滑菌落变体相比，在皱纹变体中被诱导高于基础水平，并且需要 vpsR 。这些结果表明，VpsR充当 vpsA 和 vpsL 的正向调节剂，从而正向调节EPS ETr 的产生和生物膜的形成。 展开▼

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《Journal of bacteriology》 |2001年第5期|共11页

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Fitnat H. Yildiz; Nadia A. Dolganov; Gary K. Schoolnik;
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中图分类微生物学;

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1. The quorum sensing regulator HapR downregulates the expression of the virulence gene transcription factor AphA in Vibrio cholerae by antagonizing Lrp- and VpsR-mediated activation. [J] . Lin W, Kovacikova G, Skorupski K Molecular Microbiology . 2007,第4期

机译：群体感应调节剂HapR通过拮抗Lrp和VpsR介导的激活来下调霍乱弧菌中毒力基因转录因子AphA的表达。

2. Differences in Gene Expression between the Classical and El Tor Biotypes of Vibrio cholerae O1 [J] . Sinem Beyhan, Anna D. Tischler, Andrew Camilli, Infection and immunity . 2006,第6期

机译：霍乱弧菌O1的经典和El Tor生物型之间基因表达的差异

3. Distribution of Vibrio cholerae O1 antigen biosynthesis genes among O139 and other non-O1 serogroups of Vibrio cholerae. [J] . Yamasaki S, Nair GB, Takeda Y, FEMS Microbiology Letters . 1999,第1期

机译：霍乱弧菌O1抗原生物合成基因在霍乱弧菌的O139和其他非O1血清群中的分布。

4. A method for modelling genetic regulatory networks by using evolving connectionist systems and microarray gene expression data [C] . Kasabov, N.K., Dimitrov, . 2002

机译：利用进化的连接系统和微阵列基因表达数据建立遗传调控网络的方法

5. Characterization of Cyclic-GMP-AMP Signaling and Novel Gene Networks in the Vibrio Seventh Pandemic Islands of El Tor Vibrio cholerae [D] . Severin, Geoffrey BlaineGraessley. 2020

机译：Cycrio-GMP-AMP信号传导和新型基因网络的特征在El vibrio Cholerae的vibrio第七大流行群中

6. VpsR a Member of the Response Regulators of the Two-Component Regulatory Systems Is Required for Expression of vps Biosynthesis Genes and EPSETr-Associated Phenotypes in Vibrio cholerae O1 El Tor [O] . Fitnat H. Yildiz, Nadia A. Dolganov, Gary K. Schoolnik 2001

机译：VpsR是两组分调节系统响应调节剂的成员对于霍乱弧菌O1 El Tor中vps生物合成基因和EPSETr相关表型的表达是必需的

7. VpsR, a Member of the Response Regulators of the Two-Component Regulatory Systems, Is Required for Expression of vps Biosynthesis Genes and EPSETr-Associated Phenotypes in Vibrio cholerae O1 El Tor [O] . Yildiz, Fitnat H., Dolganov, Nadia A., Schoolnik, Gary K. 2001

机译：VpsR是两组分调节系统响应调节剂的成员，对于霍乱弧菌O1 El Tor中vps生物合成基因和EPSETr相关表型的表达是必需的

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机译：多元聚合酶链反应和测试系统鉴定玻璃藻-霍乱弧菌O1菌株的方法，确定其毒性和生物毒性的方法

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VpsR, a Member of the Response Regulators of the Two-Component Regulatory Systems, Is Required for Expression ofvps Biosynthesis Genes and EPSETr-Associated Phenotypes in Vibrio cholerae O1 El Tor

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