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首页> 外文期刊>Journal of bacteriology >Rgg Regulates Growth Phase-Dependent Expression of Proteins Associated with Secondary Metabolism and Stress in Streptococcus pyogenes
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Rgg Regulates Growth Phase-Dependent Expression of Proteins Associated with Secondary Metabolism and Stress in Streptococcus pyogenes

机译：Rgg调节化脓性链球菌的次级代谢和压力相关蛋白的生长阶段依赖的表达。

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The transcriptional regulatory protein Rgg coordinates amino acid catabolism and virulence factor expression in Streptococcus pyogenes. We used a proteomic approach to compare cytoplasmic proteins isolated from S. pyogenes wild-type strain NZ131 (serotype M49) to proteins isolated from an rgg mutant strain during the exponential and stationary phases of growth. Proteins were separated by two-dimensional gel electrophoresis, and 125 protein spots of interest were identified by tandem mass spectrometry. Comparative analysis of proteins isolated from the isogenic strains revealed that growth phase-associated regulation of enzymes involved in the metabolism of arginine (ArcABC), histidine (HutI), and serine (SdhA) was abrogated in the rgg mutant strain, which synthesized the proteins in the exponential phase of growth. In contrast, the enzymes were detected only among wild-type proteins isolated from organisms in the stationary phase of growth. The differences in protein composition were correlated with previously described metabolic changes. In addition, proteins associated with thermal and oxidative stress responses, including ClpE and ClpL, were present in samples isolated from the rgg mutant strain but not in samples isolated from the wild-type strain. The rgg mutant strain was more tolerant to elevated temperature and puromycin than the wild-type strain; however, the mutant was less tolerant to paraquat. We concluded that Rgg is a global regulatory factor that contributes to growth phase-dependent synthesis of proteins associated with secondary metabolism and oxidative and thermal stress responses.
机译：转录调节蛋白Rgg负责化脓链球菌的氨基酸分解代谢和毒力因子的表达。我们使用蛋白质组学方法比较了从 S分离的胞质蛋白。化脓性野生型菌株NZ131（血清型M49）在生长的指数和固定阶段从 rgg 突变株中分离出蛋白质。通过二维凝胶电泳分离蛋白质，并通过串联质谱法鉴定125个目的蛋白质斑点。从等基因菌株分离的蛋白质的比较分析表明， rgg 废除了精氨酸（ArcABC），组氨酸（HutI）和丝氨酸（SdhA）代谢相关酶的生长阶段相关调节。突变菌株，可以在生长的指数阶段合成蛋白质。相反，仅在生长的稳定期从生物体中分离出的野生型蛋白质中检测到了酶。蛋白质组成的差异与先前描述的代谢变化相关。此外，从 rgg 突变菌株分离的样品中存在与热和氧化应激反应相关的蛋白，包括ClpE和ClpL，而从野生型菌株分离的样品中不存在。与野生型菌株相比， rgg 突变菌株对高温和嘌呤霉素的耐受性更高。然而，该突变体对百草枯的耐受性较差。我们得出的结论是，Rgg是一种全球调节因子，可促进与次级代谢，氧化和热应激反应相关的蛋白质的生长阶段依赖性合成。

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