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首页> 外文期刊>Journal of bacteriology >Mutations within the first LSGGQ motif of Ste6p cause defects in a-factor transport and mating in Saccharomyces cerevisiae.
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Mutations within the first LSGGQ motif of Ste6p cause defects in a-factor transport and mating in Saccharomyces cerevisiae.

机译：Ste6p的第一个LSGGQ基序内的突变会导致酿酒酵母a因子转运和交配中的缺陷。

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Mating between the two haploid cell types (a and alpha) of the yeast Saccharomyces cerevisiae depends upon the efficient secretion and delivery of the a- and alpha-factor pheromones to their respective target cells. However, a quantitative correlation between the level of transported a-factor and mating efficiency has never been determined. a-Factor is transported by Ste6p, a member of the ATP-binding cassette (ABC) family of transporter proteins. In this study, several missense mutations were introduced in or near the conserved LSGGQ motif within the first nucleotide-binding domain of Ste6p. Quantitation of extracellular a-factor levels indicated that these mutations caused a broad range of a-factor transport defects, and those directly within the LSGGQ motif caused the most severe defects. Overall, we observed a strong correlation between the level of transported a-factor and the mating efficiency of these strains, consistent with the role of Ste6p as the a-factor transporter. The LSGGQ mutations did not cause either a significant alteration in the steady-state level of Ste6p or a detectable change in its subcellular localization. Thus, it appears that these mutations interfere with the ability of Ste6p to transport a-factor out of the MATa cell. The possible involvement of the LSGGQ motif in transporter function is consistent with the strong conservation of this sequence motif throughout the ABC transporter superfamily.
机译：酵母酿酒酵母的两种单倍体细胞类型（α和α）之间的交配取决于α和α因子信息素向其各自靶细胞的有效分泌和传递。但是，尚未确定转运的α-因子水平与交配效率之间的定量关系。 α-因子由Ste6p转运，Ste6p是转运蛋白的ATP结合盒（ABC）家族的成员。在这项研究中，Ste6p的第一个核苷酸结合域内的保守LSGGQ基序中或附近引入了几种错义突变。定量胞外α-因子水平表明，这些突变引起广泛的α-因子转运缺陷，而直接在LSGGQ基序内的突变引起最严重的缺陷。总体而言，我们观察到转运的α-因子水平与这些菌株的交配效率之间存在很强的相关性，这与Ste6p作为α-因子转运蛋白的作用一致。 LSGGQ突变既未引起Ste6p稳态水平的显着变化，也未引起其亚细胞定位的可检测变化。因此，似乎这些突变干扰了Ste6p将α-因子转运出MATa细胞的能力。 LSGGQ基序可能参与转运蛋白功能与该序列基序在整个ABC转运蛋白超家族中的强保守性相一致。

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