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首页> 外文期刊>Journal of bacteriology >Intraspecific Variation of Unusual Phospholipids from Corynebacterium spp. Containing a Novel Fatty Acid
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Intraspecific Variation of Unusual Phospholipids from Corynebacterium spp. Containing a Novel Fatty Acid

机译：棒状杆菌属中异常磷脂的种内变异含有新型脂肪酸

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The novel fatty acid trans-9-methyl-10-octadecenoic acid was isolated from the coryneform bacterial strain LMG 3820 (previously misidentified as Arthrobacter globiformis) and identified by spectroscopic methods and chemical derivatization. This fatty acid is attached to the unusual lipid acyl phosphatidylglycerol. Five different species of this lipid type were identified; their structures were elucidated by tandem mass spectrometry and are reported here for the first time. Additionally, we identified three different cardiolipins, two bearing the novel fatty acid. The characteristic 10-methyl-octadecanoic acid was present only in phosphatidylinositol. Because of the unusual fatty acid pattern of strain LMG 3820, the 16S rDNA sequence was determined and showed regions of identity to sequences of Corynebacterium variabilis DSM 20132T and DSM 20536. All three strains possessed the novel fatty acid, identifying trans-9-methyl-10-octadecenoic acid as a potential biomarker characteristic for this taxon. Surprisingly, the fatty acid and relative abundances of phospholipids ofCorynebacterium sp. strain LMG 3820 were similar to those of the type strain but different from those of Corynebacterium variabilis DSM 20536, although all three strains possessed identical 16S rDNA sequences and strains DSM 20132T and DSM 20536 have 90.5% DNA-DNA homology. This is one of the rare cases wherein different organisms with identical 16S rDNA sequences have been observed to present recognizably different fatty acid and lipid compositions. Since methylation of a fatty acid considerably lowers the transition temperature of the corresponding lipid resulting in a more flexible cell membrane, the intraspecific variation in the lipid composition, coinciding with the morphological and Gram stain reaction variability of this species, probably offers an advantage for this species to inhabit different environmental niches.
机译：从棒状细菌菌株LMG 3820（先前误认为是 glothformis ）中分离出新型脂肪酸 trans -9-甲基-10-十八碳烯酸，并通过光谱法鉴定和化学衍生化。该脂肪酸与不寻常的脂质酰基磷脂酰甘油相连。鉴定出五种不同类型的脂质。通过串联质谱法阐明了它们的结构，并首次在此报道。此外，我们鉴定了三种不同的心磷脂，其中两种带有新型脂肪酸。仅在磷脂酰肌醇中存在特征性的10-甲基-十八烷酸。由于LMG 3820菌株具有异常的脂肪酸模式，因此确定了16S rDNA序列，并显示了与 Corynebacterium v​​ariabilis DSM 20132 T 和DSM 20536序列相同的区域。三株菌株具有新的脂肪酸，将反式 -9-甲基-10-十八碳烯酸鉴定为该分类单元的潜在生物标记特征。出乎意料的是，<棒>棒杆菌 sp。的脂肪酸和相对丰度。 LMG 3820菌株与类型菌株相似，但与 Corynebacterium v​​ariabilis DSM 20536菌株不同，尽管这三个菌株均具有相同的16S rDNA序列，菌株DSM 20132 T 和DSM 20536具有90.5％的DNA-DNA同源性。这是罕见的情况之一，其中已观察到具有相同16S rDNA序列的不同生物呈现出可识别的不同脂肪酸和脂质成分。由于脂肪酸的甲基化会大大降低相应脂质的转变温度，从而导致细胞膜更柔软，因此脂质组成的种内变化与该物种的形态学和革兰氏染色反应变异性相吻合，可能为此提供了优势物种栖息在不同的环境生态位中。

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