Analysis of the Exochelin Locus inMycobacterium smegmatis: Biosynthesis Genes Have Homology with Genes of the Peptide Synthetase Family

Shengwei Yu; Ellen Fiss; William R. Jacobs

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Analysis of the Exochelin Locus inMycobacterium smegmatis: Biosynthesis Genes Have Homology with Genes of the Peptide Synthetase Family

机译：耻垢分枝杆菌中Exochelin基因座的分析：生物合成基因与肽合成酶家族的基因具有同源性

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Mycobacteria secrete the siderophore exochelin when grown under iron-limiting conditions. In order to understand iron uptake mechanisms in mycobacteria, we have taken a genetic approach to identify those genes involved in exochelin biosynthesis and transport in Mycobacterium smegmatis. Of the 6,000 chemically mutagenized clones of M. smegmatismc2155 screened on agar plates containing chrome azural S, 19 mutants that had lost the ability to produce or secrete exochelin were identified. Thirteen of these mutants were complemented by a single M. smegmatis cosmid. Sequence analysis of this cosmid revealed nine open reading frames, three of which are homologous to genes encoding transporter proteins, which are likely involved in exochelin transport. Complementation and Tn10 mutagenesis analysis identified two new genes,fxbB and fxbC, which are required for exochelin biosynthesis. The fxbB andfxbC genes encode large proteins of 257 and 497 kDa, respectively, which are highly homologous to peptide synthetases, indicating that exochelin biosynthesis occurs by a nonribosomal mechanism.

机译：分枝杆菌在限铁条件下生长时会分泌铁载体外螯素。为了了解分枝杆菌中铁的吸收机制，我们采用了一种遗传方法来鉴定那些与耻垢分枝杆菌中exochelin生物合成和运输有关的基因。在 M的6,000个化学诱变的克隆中。在含有铬天青S的琼脂平板上筛选了耻垢包埋菌 mc 2 155，鉴定出19种丧失了产生或分泌exochelin能力的突变体。这些突变体中有13个由单个 M补充。包皮垢。该粘粒的序列分析揭示了九个开放阅读框，其中三个与编码转运蛋白的基因同源，这可能与exochelin转运有关。互补和Tn 10 诱变分析确定了exochelin生物合成所需的两个新基因 fxbB 和 fxbC 。 fxbB 和 fxbC 基因分别编码257 kDa和497 kDa的大蛋白质，与肽合成酶高度同源，表明外螯素的生物合成是通过非核糖体机制进行的。 展开▼

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《Journal of bacteriology》 |1998年第17期|共10页

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Shengwei Yu; Ellen Fiss; William R. Jacobs;
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中图分类微生物学;

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1. Exochelin genes in Mycobacterium smegmatis: identification of an ABC transporter and two non-ribosomal peptide synthetase genes. [J] . Zhu W, Arceneaux JE, Beggs ML, Molecular Microbiology . 1998,第2期

机译：耻垢分枝杆菌中的Exochelin基因：鉴定ABC转运蛋白和两个非核糖体肽合成酶基因。

2. Molecular genetic analysis reveals that a nonribosomal peptide synthetase-like (NRPS-like) gene in Aspergillus nidulans is responsible for microperfuranone biosynthesis [J] . Yeh H.-H., Chiang Y.-M., Entwistle R., Applied Microbiology and Biotechnology . 2013,第3期

机译：分子遗传学分析显示构巢曲霉中的非核糖体肽合成酶样（NRPS样）基因负责微呋喃酮的生物合成

3. Molecular genetic analysis reveals that a nonribosomal peptide synthetase-like (NRPS-like) gene in Aspergillus nidulans is responsible for microperfuranone biosynthesis [J] . Hsu-Hua Yeh, Yi-Ming Chiang, Ruth Entwistle, Applied Microbiology and Biotechnology . 2012,第3期

机译：分子遗传学分析显示构巢曲霉中的非核糖体肽合成酶样（NRPS样）基因负责微呋喃酮的生物合成

4. Nonribosomal peptide synthetase involved in the biosynthesis of cephem antibiotic [C] . China-Japan-Korea Joint Symposium on Enzyme Engineering . 2004

机译：非核糖体肽合成酶参与头孢类抗生素的生物合成

5. Diversity-generating mechanisms of non-ribosomal peptide synthetases: I. Enzymes in the biosynthesis of 3,5-dihydroxy-L-phenylglycine. II. Thioesterase domain of the surfactin synthetase. [D] . Tseng, Claire Chia-Hui. 2004

机译：非核糖体肽合成酶的多样性产生机制：I. 3,5-二羟基-L-苯基甘氨酸生物合成中的酶。二。表面活性素合成酶的硫酯酶结构域。

6. Analysis of the Exochelin Locus in Mycobacterium smegmatis: Biosynthesis Genes Have Homology with Genes of the Peptide Synthetase Family [O] . Shengwei Yu, Ellen Fiss, William R. Jacobs Jr. 1998

机译：耻垢分枝杆菌中Exochelin基因座的分析：生物合成基因与肽合成酶家族的基因具有同源性

7. Analysis of the Exochelin Locus in Mycobacterium smegmatis : Biosynthesis Genes Have Homology with Genes of the Peptide Synthetase Family [O] . Shengwei Yu, Ellen Fiss, William R. Jacobs 1998

机译：分析分枝杆菌SMEGMATIS中的exochelin基因座：生物合成基因对肽合成酶系列的基因具有同源性

8. Molecular Cloning and Sequence Analysis of the Sta58 Major Antigen Gene of Rickettsia tsutsugamushi: Sequence Homology and Antigenic Comparison of Sta58 to the 60-Kilodalton Family of Stress Proteins. [R] . Stover, C. K., Marana, D. P., Dasch, G. A., 1990

机译：恙虫病立克次体sta58主要抗原基因的克隆与序列分析：sta58与60-Kilodalton家系应激蛋白的序列同源性和抗原性比较。

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机译：控制引物退火以改善核酸扩增中退火的特异性，试剂盒，扩增DNA核酸序列和靶核酸序列或核酸混合物的方法，检测DNA与mRNA互补表达的互补性（两种）或更多核酸样品。要快速扩增cDNA靶标的片段，以扩增与mRNA cDNA互补的长丝两倍全长的cDNA群体，并用与mRNA互补的5'双重修饰的长丝进行扩增而不是序列同时鉴定一个核苷酸的VO片段，以产生一个数字印刷的gDNA DNA和一个mRNA样品中的RNA。从一个mRNA样品中鉴定一个多基因家族中的同源片段是保守的，以鉴定一个核苷酸中一个核苷酸的变异。靶核酸，用于在靶核酸中诱变，以及引发剂的用途

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Analysis of the Exochelin Locus inMycobacterium smegmatis: Biosynthesis Genes Have Homology with Genes of the Peptide Synthetase Family

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