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首页> 外文期刊>Journal of bacteriology >Complex Function for SicA, a Salmonella enterica Serovar Typhimurium Type III Secretion-Associated Chaperone
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Complex Function for SicA, a Salmonella enterica Serovar Typhimurium Type III Secretion-Associated Chaperone

机译：SicA的复杂功能，沙门氏菌血清型鼠伤寒沙门氏菌III型分泌相关的伴侣。

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Salmonella enterica encodes a type III secretion system within a pathogenicity island located at centisome 63 that is essential for virulence. All type III secretion systems require the function of a family of low-molecular-weight proteins that aid the secretion process by acting as partitioning factors and/or secretion pilots. One such protein is SicA, which is encoded immediately upstream of the type III secreted proteins SipB and SipC. We found that the absence of SicA results in the degradation of both SipB and SipC. Interestingly, in the absence of SipC, SipB was not only stable but also secreted at wild-type levels in a sicA mutant background, indicating that SicA is not required for SipB secretion. We also found that SicA is capable of binding both SipB and SipC. These results are consistent with a SicA role as a partitioning factor for SipB and SipC, thereby preventing their premature association and degradation. We also found that introduction of a sicA null mutation results in the lack of expression of SopE, another type III-secreted protein. Such an effect was shown to be transcriptional. Introduction of a loss-of-function sipC mutation into the sicAmutant background rescued sopE expression. These results indicate that the effect of sicA on sopEexpression is indirect and most likely exerted through a regulatory factor(s) partitioned by SicA from SipC. These studies therefore describe a surprisingly complex function for the Salmonella enterica type III secretion-associated chaperone SicA.
机译：肠沙门氏菌（Salmonella enterica）编码位于63号病原体的致病岛中的III型分泌系统，该系统对于毒力至关重要。所有的III型分泌系统都需要低分子量蛋白质家族的功能，这些低分子量蛋白质通过充当分配因子和/或分泌先导分子来辅助分泌过程。一种这样的蛋白质是SicA，其直接编码在III型分泌蛋白质SipB和SipC的上游。我们发现，缺少SicA会导致SipB和SipC降解。有趣的是，在没有SipC的情况下，SipB不仅稳定而且可以在 sicA 突变体背景中以野生型水平分泌，这表明SipB分泌不需要SicA。我们还发现SicA能够结合SipB和SipC。这些结果与SicA作为SipB和SipC的分配因子的作用相一致，从而防止了它们的过早结合和降解。我们还发现，引入 sicA 无效突变会导致缺乏另一种III型分泌蛋白SopE的表达。这种作用被证明是转录的。将功能丧失的 sipC 突变引入 sicA 突变背景拯救的 sopE 表达中。这些结果表明， sicA 对 sopE 表达的影响是间接的，并且很可能是由SicA从SipC中分离出的调节因子发挥的。因此，这些研究描述了 Salmonella enterica III型分泌相关伴侣分子SicA令人惊讶的复杂功能。

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