...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Autophagy and the cvt Pathway Both Depend onAUT9
【24h】

Autophagy and the cvt Pathway Both Depend onAUT9

机译：自噬和cvt通路均取决于AUT9

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In growing cells of the yeast Saccharomyces cerevisiae, proaminopeptidase I reaches the vacuole via the selective cytoplasm-to-vacuole targeting (cvt) pathway. During nutrient limitation, autophagy is also responsible for the transport of proaminopeptidase I. These two nonclassical protein transport pathways to the vacuole are distinct in their characteristics but in large part use identical components. We expanded our initial screen foraut ? mutants and isolated aut9-1cells, which show a defect in both pathways, the vacuolar targeting of proaminopeptidase I and autophagy. By complementation of the sporulation defect of homocygous diploid aut9-1 mutant cells with a genomic library, in this study we identified and characterized the AUT9 gene, which is allelic withCVT7. aut9-deficient cells have no obvious defects in growth on rich media, vacuolar biogenesis, and acidification, but like other mutant cells with a defect in autophagy, they exhibit a reduced survival rate and reduced total protein turnover during starvation. Aut9p is the first putative integral membrane protein essential for autophagy. A biologically active green fluorescent protein-Aut9 fusion protein was visualized at punctate structures in the cytosol of growing cells.
机译：在酵母酿酒酵母（Saccharomyces cerevisiae）的生长细胞中，前氨基肽酶I通过选择性的细胞质-真空靶向（cvt）途径到达液泡。在营养限制期间，自噬还负责原氨肽酶I的运输。这两种非经典的蛋白向液泡的运输途径具有不同的特征，但大部分使用相同的成分。我们扩展了对 aut ？突变体和分离的 aut9-1 细胞的初始筛选，这些细胞在两种途径中均存在缺陷，即液泡靶向原氨基肽酶我和自噬。通过用基因组文库对同源二倍体 aut9-1 突变细胞的孢子形成缺陷进行补充，在本研究中，我们鉴定并鉴定了与等位基因的 AUT9 基因。 > CVT7。缺乏aut9 的细胞在富培养基，液泡生物发生和酸化方面没有明显的生长缺陷，但是像其他具有自噬缺陷的突变细胞一样，它们在饥饿期间显示出降低的存活率和总蛋白质更新。 Aut9p是自噬必不可少的第一个推定的整合膜蛋白。在生长细胞的胞质溶胶中的点状结构处观察到了具有生物活性的绿色荧光蛋白-Aut9融合蛋白。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号