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首页> 外文期刊>Journal of bacteriology >A Green Nonsulfur Bacterium, Dehalococcoides ethenogenes, with the LexA Binding Sequence Found in Gram-Positive Organisms
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A Green Nonsulfur Bacterium, Dehalococcoides ethenogenes, with the LexA Binding Sequence Found in Gram-Positive Organisms

机译:在革兰氏阳性生物中发现具有LexA结合序列的绿色无硫细菌Dehalococcoides ethenogenes。

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Dehalococcoides ethenogenes is a member of the physiologically diverse division of green nonsulfur bacteria. Using a TBLASTN search, the D. ethenogenes lexA gene has been identified, cloned, and expressed and its protein has been purified. Mobility shift assays revealed that the D. ethenogenes LexA protein specifically binds to both its own promoter and that of the uvrA gene, but not to the recA promoter. Our results demonstrate that the D. ethenogenes LexA binding site is GAACN4GTTC, which is identical to that found in gram-positive bacteria. In agreement with this fact, the Bacillus subtilis DinR protein binds specifically to the D. ethenogenes LexA operator. This constitutes the first non-gram-positive bacterium exhibiting a LexA binding site identical to that of B. subtilis.
机译: Dehalococcoides ethenogenes 是绿色无硫细菌在生理上的不同组成部分。使用TBLASTN搜索, D。已鉴定,克隆并表达了埃塞氏菌lexA 基因,并纯化了其蛋白质。迁移率变化分析表明, D。乙二胺 LexA蛋白与它自己的启动子和 uvrA 基因的启动子特异性结合,但不与 recA 启动子结合。我们的结果表明 D。乙炔的LexA结合位点是GAACN 4 GTTC,与革兰氏阳性细菌中的相同。与这个事实一致的是,枯草芽孢杆菌 DinR蛋白与 D特异性结合。 ethenogenes LexA运算符。这构成了第一个非革兰氏阳性细菌,表现出与 B相同的LexA结合位点。枯草杆菌。

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