...
首页> 外文期刊>Journal of bacteriology >Lack of Strand Bias in UV-Induced Mutagenesis in Escherichia coli
【24h】

Lack of Strand Bias in UV-Induced Mutagenesis in Escherichia coli

机译:紫外线诱导的大肠杆菌诱变中链偏倚的缺乏。

获取原文
           

摘要

We have investigated whether UV-induced mutations are created with equal efficiency on the leading and lagging strands of DNA replication. We employed an assay system that permits measurement of mutagenesis in the lacZ gene in pairs of near-identical strains. Within each pair, the strains differ only in the orientation of the lacZ gene with respect to the origin of DNA replication. Depending on this orientation, any lacZ target sequence will be replicated in one orientation as a leading strand and as a lagging strand in the other orientation. In contrast to previous results obtained for mutations resulting from spontaneous replication errors or mutations resulting from the spontaneous SOS mutator effect, measurements of UV-induced mutagenesis in uvrA strains fail to show significant differences between the two target orientations. These data suggest that SOS-mediated mutagenic translesion synthesis on the Escherichia coli chromosome may occur with equal or similar probability on leading and lagging strands.
机译:我们研究了紫外线诱导的突变是否在DNA复制的前导链和滞后链上均以相同的效率产生。我们采用了一种检测系统,该系统可以测量成对的近乎相同的菌株对 lacZ 基因的诱变作用。在每一对中,毒株仅在 lacZ 基因相对于DNA复制起点的方向上有所不同。取决于该方向,任何 靶序列将在一个方向上作为前导链和作为落后链在另一方向上复制。与先前因自发复制错误导致的突变或因自发SOS突变体效应导致的突变获得的先前结果相反,对 uvrA 菌株中紫外线诱导的诱变的测量未能显示出两个靶标方向之间的显着差异。这些数据表明,SOS介导的大肠杆菌染色体上的诱变转移合成可能以相同或相似的概率发生在前导链和滞后链上。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号