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首页> 外文期刊>Journal of bacteriology >Control by A-Factor of a Metalloendopeptidase Gene Involved in Aerial Mycelium Formation in Streptomyces griseus
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Control by A-Factor of a Metalloendopeptidase Gene Involved in Aerial Mycelium Formation in Streptomyces griseus

机译：灰链霉菌空中菌丝形成的金属内肽酶基因的A因子控制

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In Streptomyces griseus, A-factor (2-isocapryloyl-3R-hydroxymethyl-γ-butyrolactone) switches on aerial mycelium formation and secondary metabolite biosynthesis. An A-factor-dependent transcriptional activator, AdpA, activates multiple genes required for morphological development and secondary metabolism in a programmed manner. A region upstream of a zinc-containing metalloendopeptidase gene (sgmA) was found among the DNA fragments that had been isolated as AdpA-binding sites. The primary product of sgmA consisted of N-terminal pre, N-terminal pro, mature, and C-terminal pro regions. sgmA was transcribed in an AdpA-dependent manner, and its transcription was markedly enhanced at the timing of aerial mycelium formation. AdpA bound two sites in the region upstream of the sgmA promoter; one was at about nucleotide position ?60 (A site) with respect to the transcriptional start point of sgmA, and the other was at about position ?260 (B site), as determined by DNase I footprinting. Transcriptional analysis with mutated promoters showed that the A site was essential for the switching on of sgmA transcription and that the B site was necessary for the marked enhancement of transcription at the timing of aerial mycelium formation. Disruption of the chromosomal sgmA gene resulted in a delay in aerial hypha formation by half a day. SgmA is therefore suggested to be associated with the programmed morphological development of Streptomyces, in which this peptidase, perhaps together with other hydrolytic enzymes, plays a role in the degradation of proteins in substrate hyphae for reuse in aerial hypha formation.
机译：在链霉菌 灰霉菌中，A因子（2-异辛酰基-3 R -羟甲基-γ-丁内酯）会改变气生菌丝的形成和次级代谢产物生物合成。 A因子依赖性转录激活因子AdpA以编程方式激活形态发育和次级代谢所需的多个基因。在已分离出作为AdpA结合位点的DNA片段中，发现了含锌金属内肽酶基因（ sgmA ）上游的区域。 sgmA 的主要产物由N端前，N端前，成熟和C端前区组成。 sgmA 以AdpA依赖性方式转录，在气生菌丝形成时其转录明显增强。 AdpA结合了 sgmA 启动子上游区域中的两个位点。一个是相对于 sgmA 转录起始点的大约60位核苷酸（A位），另一个是大约260位（B位），这是通过DNase I足迹确定的。用突变的启动子进行的转录分析表明，A位点对于 sgmA 转录的开启是必不可少的，而B位点对于在气生菌丝体形成时显着增强转录是必需的。染色体 sgmA 基因的破坏导致空中菌丝形成延迟了半天。因此，建议将SgmA与链霉菌（Streptomyces）的编程形态学发展相关，其中该肽酶，也许与其他水解酶一起，在底物菌丝中的蛋白质降解中起作用，以便在空中菌丝中重复使用。编队。

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