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首页> 外文期刊>Journal of bacteriology >Acetic Acid Induces Expression of the Staphylococcus aureus cidABC and lrgAB Murein Hydrolase Regulator Operons
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Acetic Acid Induces Expression of the Staphylococcus aureus cidABC and lrgAB Murein Hydrolase Regulator Operons

机译：乙酸诱导金黄色葡萄球菌cidABC和lrgAB Murein水解酶调节子的表达。

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The Staphylococcus aureus lrg and cid operons encode homologous proteins that regulate extracellular murein hydrolase activity and penicillin tolerance in a diametrically opposing manner. Although their specific regulatory functions remain unknown, it has been postulated that the functions of CidA and LrgA are analogous to those of bacteriophage holins and antiholins, respectively, and that these proteins serve as molecular control elements of bacterial programmed cell death. Although these studies demonstrated that cidBC transcription is abundant in σB-proficient strains, cidABC transcription was only minimally expressed under standard growth conditions. In this study, we demonstrate that cidABC and lrgAB transcription in the clinical isolate UAMS-1 is induced by growth in the presence of 35 mM glucose and that this enhances murein hydrolase activity and decreases tolerance to vancomycin and rifampin. The effect of glucose on murein hydrolase activity was not observed in the cidA mutant, indicating that the induction of this activity was dependent on enhanced cidABC expression. Furthermore, we demonstrate that the effects of glucose on cidABC and lrgAB transcription are mediated by the generation of acetic acid produced by the metabolism of this and other carbon sources. These results shed new light on the control of the S. aureus cidABC and lrgAB genes and demonstrate that these operons, as well as murein hydrolase activity and antibiotic tolerance, are responsive to carbohydrate metabolism.
机译：金黄色葡萄球菌lrg 和 cid 操纵子编码同源蛋白，它们以截然相反的方式调节细胞外murein水解酶活性和青霉素耐受性。尽管它们的特定调节功能仍然未知，但据推测CidA和LrgA的功能分别类似于噬菌体Holins和抗Holins，并且这些蛋白质可作为细菌程序性细胞死亡的分子控制元件。尽管这些研究表明 cidBC 转录在σ B 精通菌株中丰富，但 cidABC 转录在标准生长条件下仅极少表达。在这项研究中，我们证明了临床分离株UAMS-1中的 cidABC 和 lrgAB 转录是由在35 mM葡萄糖存在下的生长诱导的，并且这增强了murein水解酶的活性。并降低了对万古霉素和利福平的耐受性。在 cidA 突变体中未观察到葡萄糖对murein水解酶活性的影响，表明该活性的诱导取决于增强的 cidABC 表达。此外，我们证明了葡萄糖对 cidABC 和 lrgAB 转录的影响是由乙酸和其他碳源代谢产生的乙酸介导的。这些结果为 S的控制提供了新的思路。金黄色葡萄球菌的cidABC 和 lrgAB 基因，并证明这些操纵子，以及murein水解酶的活性和抗生素耐受性对碳水化合物的代谢有反应。

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