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首页> 外文期刊>Journal of bacteriology >Sequencing of Multiple Clostridial Genomes Related to Biomass Conversion and Biofuel Production
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Sequencing of Multiple Clostridial Genomes Related to Biomass Conversion and Biofuel Production

机译：与生物质转化和生物燃料生产相关的多个梭菌基因组的测序

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Modern methods to develop microbe-based biomass conversion processes require a system-level understanding of the microbes involved. Clostridium species have long been recognized as ideal candidates for processes involving biomass conversion and production of various biofuels and other industrial products. To expand the knowledge base for clostridial species relevant to current biofuel production efforts, we have sequenced the genomes of 20 species spanning multiple genera. The majority of species sequenced fall within the class III cellulosome-encoding Clostridium and the class V saccharolytic Thermoanaerobacteraceae. Species were chosen based on representation in the experimental literature as model organisms, ability to degrade cellulosic biomass either by free enzymes or by cellulosomes, ability to rapidly ferment hexose and pentose sugars to ethanol, and ability to ferment synthesis gas to ethanol. The sequenced strains significantly increase the number of noncommensalonpathogenic clostridial species and provide a key foundation for future studies of biomass conversion, cellulosome composition, and clostridial systems biology.
机译：开发基于微生物的生物质转化过程的现代方法需要对涉及的微生物有系统的了解。梭菌属菌种一直以来被认为是涉及生物质转化以及各种生物燃料和其他工业产品生产过程的理想候选者。为了扩展与当前生物燃料生产工作相关的梭菌物种的知识库，我们对跨多个属的20种物种的基因组进行了测序。测序的大多数物种都属于编码III类纤维素酶的 Clostridium 和V类糖酵解 Thermoanaerobacteraceae 。根据实验文献中作为模型生物的代表，通过游离酶或纤维素酶降解纤维素生物质的能力，将己糖和戊糖快速发酵为乙醇的能力以及将合成气发酵为乙醇的能力来选择物种。测序菌株显着增加了非共生/非致病性梭菌种类的数量，并为生物质转化，纤维素体组成和梭菌系统生物学的未来研究提供了重要基础。

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