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首页> 外文期刊>Journal of bacteriology >Promoter Substitution and Deletion Analysis of Upstream Region Required for rpoS Translational Regulation
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Promoter Substitution and Deletion Analysis of Upstream Region Required for rpoS Translational Regulation

机译：rpoS转化调控所需的上游区域的启动子取代和缺失分析

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The RpoS sigma factor of enteric bacteria is required for the increased expression of a number of genes that are induced during nutrient limitation and growth into stationary phase and in response to high osmolarity. RpoS is also a virulence factor for several pathogenic species, including Salmonella typhimurium. The activity of RpoS is regulated at both the level of synthesis and protein turnover. Here we investigate the posttranscriptional control of RpoS synthesis by using rpoS-lac protein and operon fusions. Substitution of the native rpoS promoters with the tac orlac UV5 promoters allowed essentially normal regulation after growth into stationary phase in rich medium or after osmotic challenge. Regulation of these fusions required the function ofhfq, encoding the RNA-binding protein host factor I (HF-I). Short deletions from the 5′ end of the rpoS transcript did not affect regulation very much; however, a larger deletion mutation that still retains 220 bp upstream of the rpoS ATG codon, including a proposed antisense element inhibitory for rpoStranslation, was no longer regulated by HF-I. Several models for regulation of rpoS expression by HF-I are discussed.
机译：肠道细菌的RpoS sigma因子是在营养限制和生长成固定相以及对高渗透压的反应过程中诱导的许多基因表达增加所必需的。 RpoS还是多种病原体的毒力因子，包括鼠伤寒沙门氏菌。 RpoS的活性受合成水平和蛋白质更新的调节。在这里，我们通过使用 rpoS-lac 蛋白和操纵子融合体来研究RpoS合成的转录后控制。用 tac 或 lac UV5启动子替代天然的 rpoS 启动子后，在丰富的培养基中生长成固定相后或在渗透挑战后基本上可以正常调节。这些融合的调节需要 hfq 的功能，它们编码RNA结合蛋白宿主因子I（HF-1）。 rpoS 转录本5'端的短缺失并没有很大地影响调节。然而，更大的缺失突变仍保留在 rpoS ATG密码子上游220 bp处，包括拟议的抑制 rpoS 翻译的反义元件，不再受HF-I的调控。 。讨论了几种通过HF-1调控 rpoS 表达的模型。

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