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首页> 外文期刊>Journal of bacteriology >Physical Mapping and Functional Assignment of the Geranylgeranyl-Bacteriochlorophyll Reductase Gene, bchP, of Rhodobacter sphaeroides
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Physical Mapping and Functional Assignment of the Geranylgeranyl-Bacteriochlorophyll Reductase Gene, bchP, of Rhodobacter sphaeroides

机译：球形球形红发菌的Geranylgeranyl-细菌叶绿素还原酶基因bchP的物理定位和功能分配

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The bacteriochlorophyll of the purple photosynthetic bacteriumRhodobacter sphaeroides is esterified with phytol. The presence of this alcohol moiety is essential for the correct assembly of the photosynthetic apparatus. Despite this, and the fact thatR. sphaeroides is widely used for the study of structure-function relationships in photosynthesis, the molecular genetics of the steps in which the alcohol is added and modified have not previously been investigated in this organism. Sequencing near the center of the photosynthesis gene cluster has now revealed the existence of an open reading frame encoding a putative 394-amino-acid polypeptide displaying strong homology with the products of a number of genes from other photosynthetic organisms, each proposed to be responsible for the reduction of the alcohol moiety of (bacterio)chlorophyll to phytol. An R. sphaeroidestransposon mutant in this gene, bchP, possessed a structurally modified photosystem assembled with bacteriochlorophyll esterified with geranylgeraniol, rather than with phytol, implying that the product of this gene was geranylgeranyl-bacteriochlorophyll reductase. This identification was confirmed by the performance of in vitro assays using heterologously expressed protein, providing the first direct demonstration of the activity of a bchP gene product.
机译：紫色光合细菌球形芽孢杆菌的叶绿素被植醇酯化。该醇部分的存在对于光合装置的正确组装是必不可少的。尽管如此，事实是 R。 sphaeroides 被广泛用于研究光合作用中的结构-功能关系，此前尚未在该生物中研究添加和修饰醇的步骤的分子遗传学。现在，在光合作用基因簇中心附近进行测序揭示了一个开放阅读框的存在，该阅读框编码一个推定的394个氨基酸多肽，该多肽与来自其他光合作用生物的许多基因的产物表现出强烈的同源性，每个基因都被认为与（细菌）叶绿素的醇部分还原为植醇。一个 R。该基因中的sphaeroides 转座子突变体 bchP 具有结构修饰的光系统，该系统装配有被香叶基香叶醇而不是植醇酯化的细菌叶绿素，暗示该基因的产物是香叶基香叶基-细菌叶绿素还原酶。通过使用异源表达的蛋白质进行体外测定的性能，证实了这种鉴定，这是 bchP 基因产物活性的首次直接证明。

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