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首页> 外文期刊>Journal of bacteriology >Cell Density-Dependent Starvation Survival ofRhizobium leguminosarum bv. phaseoli: Identification of the Role of an N- Acyl Homoserine Lactone in Adaptation to Stationary-Phase Survival
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Cell Density-Dependent Starvation Survival ofRhizobium leguminosarum bv. phaseoli: Identification of the Role of an N- Acyl Homoserine Lactone in Adaptation to Stationary-Phase Survival

机译：豆根瘤菌bv的细胞密度依赖性饥饿存活。菜豆素：鉴定N-酰基高丝氨酸内酯在适应平稳期生存中的作用

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The cell density dependence of stationary-phase survival ofRhizobium leguminosarum has been investigated. Following starvation by exhaustion of carbon or nitrogen, but not of phosphorus, the survival of cultures was dependent on the cell density at entry into stationary phase. High-density cultures survived with little or no loss of viability over a 20-day period in stationary phase. In contrast, low-density cultures lost viability rapidly but consisted of a heterogeneous population, a small fraction of which successfully adapted and eventually formed a stable, surviving population. The threshold density above which the cultures survived successfully in stationary phase was dependent on the growth conditions and the strain used. We took advantage of the fact that R. leguminosarumsurvives poorly following starvation by resuspension in carbon-free medium to demonstrate that cell density-dependent survival was mediated by a component accumulating in the growth medium. The effects of this medium component on survival in resuspension assays could be mimicked by an N-acyl homoserine lactone,N-(3R-hydroxy-7-cis-tetradecanoyl)-l-homoserine lactone, previously demonstrated to have a role in controlling cell density-dependent phenomena in R. leguminosarum. The Sym plasmids pRP2JI and pRL1JI were found to be essential for the production of the extracellular factor, which could also be made inEscherichia coli carrying the cosmid clone pIJ1086 containing a specific region of pRL1JI.
机译：研究了豆科植物根瘤菌根瘤菌固定期存活的细胞密度依赖性。由于碳或氮而不是磷的缺乏而饥饿后，培养物的存活取决于进入静止期的细胞密度。高密度培养物在静止期的20天时间内几乎没有存活，甚至没有存活。相反，低密度培养物迅速丧失了生存能力，但由异质种群组成，其中一小部分成功地适应并最终形成了一个稳定的存活种群。高于阈值密度的培养物在固定相中成功存活的阈值密度取决于生长条件和所用菌株。我们利用了 R。在无碳培养基中重悬饥饿后，豆科植物的存活率很低，这表明细胞密度依赖性存活是由生长培养基中积累的一种成分介导的。 N -酰基高丝氨酸内酯， N -（3 R -羟基-7- 顺式-十四烷酰基）-1-高丝氨酸内酯，先前证明在控制 R中细胞密度依赖性现象中具有作用。豆科恐龙。发现Sym质粒pRP2JI和pRL1JI对于细胞外因子的产生是必不可少的，这也可以在带有含有pRL1JI特定区域的粘粒克隆pIJ1086的大肠杆菌中制备。

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