...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Similarities between the antABC-Encoded Anthranilate Dioxygenase and the benABC-Encoded Benzoate Dioxygenase of Acinetobacter sp. Strain ADP1
【24h】

Similarities between the antABC-Encoded Anthranilate Dioxygenase and the benABC-Encoded Benzoate Dioxygenase of Acinetobacter sp. Strain ADP1

机译：antABC编码的邻氨基苯甲酸双加氧酶与不动杆菌编码的benABC编码的苯甲酸酯双加氧酶之间的相似性。菌株ADP1

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Acinetobacter sp. strain ADP1 can use benzoate or anthranilate as a sole carbon source. These structurally similar compounds are independently converted to catechol, allowing further degradation to proceed via the β-ketoadipate pathway. In this study, the first step in anthranilate catabolism was characterized. A mutant unable to grow on anthranilate, ACN26, was selected. The sequence of a wild-type DNA fragment that restored growth revealed theantABC genes, encoding 54-, 19-, and 39-kDa proteins, respectively. The deduced AntABC sequences were homologous to those of class IB multicomponent aromatic ring-dihydroxylating enzymes, including the dioxygenase that initiates benzoate catabolism. Expression of antABC in Escherichia coli, a bacterium that normally does not degrade anthranilate, enabled the conversion of anthranilate to catechol. Unlike benzoate dioxygenase (BenABC), anthranilate dioxygenase (AntABC) catalyzed catechol formation without requiring a dehydrogenase. In Acinetobacter mutants,benC substituted for antC during growth on anthranilate, suggesting relatively broad substrate specificity of the BenC reductase, which transfers electrons from NADH to the terminal oxygenase. In contrast, the benAB genes did not substitute for antAB. An antA point mutation in ACN26 prevented anthranilate degradation, and this mutation was independent of a mucK mutation in the same strain that prevented exogenous muconate degradation. Anthranilate induced expression of antA, although no associated transcriptional regulators were identified. Disruption of three open reading frames in the immediate vicinity ofantABC did not prevent the use of anthranilate as a sole carbon source. TheantABC genes were mapped on the ADP1 chromosome and were not linked to the two known supraoperonic gene clusters involved in aromatic compound degradation.
机译：不动杆菌 sp。菌株ADP1可以使用苯甲酸酯或邻氨基苯甲酸酯作为唯一碳源。这些结构相似的化合物独立地转化为儿茶酚，从而允许进一步降解通过β-酮己二酸酯途径进行。在这项研究中，表征了邻氨基苯甲酸分解代谢的第一步。选择了不能在邻氨基苯甲酸上生长的突变体ACN26。恢复生长的野生型DNA片段序列揭示了 antABC 基因，分别编码54-，19-和39-kDa蛋白。推导的AntABC序列与IB类多组分芳香环二羟基化酶（包括引发苯甲酸酯分解代谢的双加氧酶）的序列同源。 antABC 在大肠杆菌中的表达，该细菌通常不会降解邻氨基苯甲酸，可将邻氨基苯甲酸转化为邻苯二酚。与苯甲酸酯双加氧酶（BenABC）不同，邻氨基苯甲酸双加氧酶（AntABC）催化邻苯二酚的形成，而无需脱氢酶。在不动杆菌突变体中，在邻氨基苯甲酸上生长期间， benC 替代了 antC ，这表明BenC还原酶具有相对广泛的底物特异性，该酶将电子从NADH转移至末端加氧酶。相反， benAB 基因不能替代 antAB 。 ACN26中的 antA 点突变可防止邻氨基苯甲酸降解，并且该突变与防止外源性粘液降解的同一菌株中的 mucK 突变无关。邻氨基苯甲酸诱导 antA 的表达，尽管未发现相关的转录调节因子。破坏 antABC 附近的三个开放阅读框并不能阻止邻氨基苯甲酸作为唯一碳源。 antABC 基因被定位在ADP1染色体上，并且没有与参与芳香族化合物降解的两个已知的超操纵基因簇相关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号