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首页> 外文期刊>Journal of bacteriology >Characterization of IS1547, a New Member of the IS900 Family in the Mycobacterium tuberculosis Complex, and Its Association with IS6110
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Characterization of IS1547, a New Member of the IS900 Family in the Mycobacterium tuberculosis Complex, and Its Association with IS6110

机译：IS1547（结核分枝杆菌复合物中IS900家族的新成员）的表征及其与IS6110的关联

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摘要

Unlike classically defined insertion sequence (IS) elements, which are delimited by their inverted terminal repeats, some IS elements do not have inverted terminal repeats. Among this group of atypical IS elements, IS116, IS900, IS901, and IS1110 have been proposed as members of the IS900 family of elements, not only because they do not have inverted terminal repeats but also because they share other features such as homologous transposases and particular insertion sites. In this study, we report a newly identified IS sequence, IS1547, which was first identified in a clinical isolate of Mycobacterium tuberculosis. Its structure, insertion site, and putative transposase all conform with the conventions of the IS900family, suggesting that it is a new member of this family. IS1547 was detected only in isolates of the M. tuberculosis complex, where it had highly polymorphic restriction fragment length polymorphism patterns, suggesting that it may be a useful genetic marker for identifying isolates of the M. tuberculosis complex and for distinguishing different strains ofM. tuberculosis. ipl is a preferential locus for IS6110 insertion where there are eight known different insertion sites for IS6110. Surprisingly, the DNA sequence of ipl is now known to be a part of IS1547, meaning that IS1547 is a preferential site for IS6110 insertion.
机译：与经典定义的插入序列（IS）元素（由其反向末端重复序列来界定）不同，某些IS元素没有反向末端重复序列。在这组非典型IS元素中，IS 116 ，IS 900 ，IS 901 和IS 1110 之所以提议将其作为IS 900 家族的成员，不仅因为它们没有反向末端重复序列，而且还因为它们具有其他功能，例如同源转座酶和特定的插入位点。在这项研究中，我们报告了一个新鉴定的IS序列IS 1547 ，该序列首先在结核分枝杆菌的临床分离株中鉴定。它的结构，插入位点和推定的转座酶均符合IS 900 家族的惯例，表明它是该家族的新成员。仅在 M的分离物中检测到IS 1547 。结核病复合物，它具有高度多态性的限制性片段长度多态性模式，表明它可能是鉴定 M分离物的有用遗传标记。结核复合物并用于区分不同的 M菌株。结核病。 ipl 是IS 6110 插入的优先位点，其中有八个已知的IS 6110 插入位点。令人惊讶的是，现在已知 ipl 的DNA序列是IS 1547 的一部分，这意味着IS 1547 是IS 的优先位点em> 6110 插入。

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