...
首页> 外文期刊>Journal of bacteriology >Two Roles for the Leucine-Responsive Regulatory Protein in Expression of the Alanine Catabolic Operon (dadAB) inKlebsiella aerogenes
【24h】

Two Roles for the Leucine-Responsive Regulatory Protein in Expression of the Alanine Catabolic Operon (dadAB) inKlebsiella aerogenes

机译:亮氨酸反应性调节蛋白在产酸克雷伯菌中丙氨酸分解代谢操纵子(dadAB)表达中的两个作用。

获取原文
           

摘要

The lrp gene, which codes for the leucine-responsive regulatory protein (Lrp), was cloned from Klebsiella aerogenes W70. The DNA sequence was determined, and the clone was used to create a disruption of the lrp gene. The lack of functional Lrp led to an increased expression of the alanine catabolic operon (dad) in the absence of the inducerl-alanine but also to a decreased expression of the operon in the presence of l-alanine. Thus, Lrp is both a repressor and activator of dad expression. Lrp is also necessary for glutamate synthase formation but not for the formation of two other enzymes controlled by the nitrogen regulatory (Ntr) system, glutamate dehydrogenase and histidase.
机译:编码亮氨酸反应性调节蛋白(Lrp)的 lrp 基因是从产气杆菌(Klebsiella aerogenes) W70克隆的。确定了DNA序列,并将该克隆用于破坏 lrp 基因。缺乏功能性Lrp导致在没有诱导丙氨酸的情况下丙氨酸分解代谢操纵子( dad )的表达增加,但在存在1-丙氨酸的情况下导致操纵子的表达减少。因此,Lrp既是 dad 表达的阻遏物又是激活物。 Lrp对于谷氨酸合酶的形成也是必需的,但对于由氮调节(Ntr)系统控制的其他两种酶(谷氨酸脱氢酶和组氨酸酶)则不是必需的。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号