Topology of RbsC, a Membrane Component of the Ribose Transporter, Belonging to the AraH Superfamily

Yongkyu Park; Chankyu Park

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Topology of RbsC, a Membrane Component of the Ribose Transporter, Belonging to the AraH Superfamily

机译：RbsC的拓扑结构，RbsC是核糖转运蛋白的膜成分，属于AraH超家族

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RbsC of Escherichia coli is the hydrophobic membrane component of ribose uptake system classified as the ATP-binding cassette transporter. To understand the structure and function of RbsC, its transmembrane topology was investigated by using 64 RbsC-PhoA fusions isolated either specifically or randomly. In order to confirm the cytoplasmic location of the short C-terminal region (5 amino acids), inside-out or right-side-out membrane vesicles were generated, and the C-terminal region was found to be digested by carboxypeptidase A only in inside-out vesicles. This result is consistent with the model, based on the results of alkaline phosphatase fusions, in which the protein traverses the membrane six times and the N and C termini are exposed to the cytoplasm.

机译：大肠埃希菌的RbsC是核糖摄取系统的疏水膜成分，被分类为ATP结合盒转运蛋白。为了了解RbsC的结构和功能，通过使用64种RbsC-PhoA融合蛋白（特异或随机分离）研究了其跨膜拓扑。为了确认短C末端区域（5个氨基酸）的胞质位置，产生了由内向外或从右侧向外的膜囊泡，并且发现C末端区域仅被羧肽酶A消化。由内而外的囊泡。基于碱性磷酸酶融合的结果，该结果与该模型相符，其中蛋白质横穿膜六次，N和C末端暴露于细胞质。

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《Journal of bacteriology》 |1999年第3期|共4页
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Yongkyu Park; Chankyu Park;
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1. Topology of RbsC, the Membrane Component of the Escherichia coli Ribose Transporter [J] . Jeffrey B. Stewart, Mark A. Hermodson Journal of bacteriology . 2003,第17期

机译：RbsC的拓扑结构，大肠杆菌核糖转运蛋白的膜成分
2. Membrane topology of the Escherichia coli gamma-aminobutyrate transporter: implications on the topography and mechanism of prokaryotic and eukaryotic transporters from the APC superfamily. [J] . Hu LA, King SC The Biochemical Journal . 1998,第Pta1期

机译：大肠杆菌γ-氨基丁酸转运蛋白的膜拓扑：对来自APC超家族的原核和真核转运蛋白的拓扑结构和机制的影响。
3. Membrane topology of the Escherichia coli gamma-aminobutyrate transporter: implications on the topography and mechanism of prokaryotic and eukaryotic transporters from the APC superfamily [J] . Hu LA., King SC. The Biochemical Journal . 1998,第Pta1期

机译：大肠杆菌γ-氨基丁酸转运蛋白的膜拓扑：对来自APC超家族的原核和真核转运蛋白的拓扑结构和机制的影响
4. Reconstruction of the evolutionary history of the DHA1 family of yeast multidrug resistance transporters of the major facilitator superfamily [C] . Dias Paulo Jorge, Sa-Correia Isabel 1st Portuguese Meeting in Bioengineering . 2011

机译：重建主要促进者超家族的酵母多药耐药转运蛋白DHA1家族的进化史
5. The Francisella tularensis Phagosomal Transporter Subfamily of Major Facilitator Superfamily Transporters is a Critical Determinant of Pathogenesis and Virulence and Modulates the Host Immune Response [D] . Marohn, Mark E. 2012

机译：主要促进者超家族转运蛋白的弗朗西斯菌tularensis吞噬体转运蛋白亚家族是发病机理和毒力的关键决定因素，并调节宿主的免疫反应。
6. Note: Topology of RbsC a Membrane Component of the Ribose Transporter Belonging to the AraH Superfamily [O] . Yongkyu Park, Chankyu Park 1999

机译：注意：RbsC的拓扑结构是核糖转运蛋白的膜成分属于AraH超家族
7. Topology of RbsC, the Membrane Component of the Escherichia coli Ribose Transporter† [O] . Stewart, Jeffrey B., Hermodson, Mark A. 2003

机译：RbsC的拓扑结构，即大肠杆菌核糖转运蛋白的膜成分†

Topology of RbsC, a Membrane Component of the Ribose Transporter, Belonging to the AraH Superfamily

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