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首页> 外文期刊>Journal of bacteriology >The N-Acetyltransferase RimJ Responds to Environmental Stimuli To Repress pap Fimbrial Transcription in Escherichia coli
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The N-Acetyltransferase RimJ Responds to Environmental Stimuli To Repress pap Fimbrial Transcription in Escherichia coli

机译：N-乙酰基转移酶RimJ响应环境刺激，以抑制大肠杆菌中的子叶膜转录。

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In uropathogenic Escherichia coli, P pili (Pap) facilitate binding to host epithelial cells and subsequent colonization. Whereas P pili can be produced at 37°C, the expression of these fimbriae is suppressed at 23°C. Previously, insertion mutations in rimJ, a gene encoding the N-terminal acetyltransferase of ribosomal protein S5, were shown to disrupt this thermoregulatory response, allowing papBA transcription at low temperature. In this study, we created an in-frame deletion of rimJ. This deletion relieved the repressive effects not only of low temperature but also of rich (Luria-Bertani [LB]) medium and glucose on papBA transcription, indicating that RimJ modulates papBA transcription in response to multiple environmental stimuli. papI transcription was also shown to be regulated by RimJ. papBA transcription is also controlled by a phase variation mechanism. We demonstrated that the regulators necessary to establish a phase ON state—PapI, PapB, Dam, Lrp, and cyclic AMP-CAP-are still required for papBA transcription in a rimJ mutant strain. rimJ mutations increase the rate at which bacteria transition into the phase ON state, indicating that RimJ inhibits the phase OFF→ON transition. A ΔrimJ hns651 mutant is viable on LB medium but not on minimal medium. This synthetic lethality, along with transcriptional analyses, indicates that RimJ and H-NS work through separate pathways to control papBA transcription. Mutations in rimJ do not greatly influence the transcription of the fan, daa, or fim operon, suggesting that RimJ may be a pap-specific regulator. Overexpression of rimJ under conditions repressive for papBA transcription complements the ΔrimJ mutation but has little effect on transcription under activating conditions, indicating that the ability of RimJ to regulate transcription is environmentally controlled.
机译：在尿路致病性大肠埃希菌中，P pili（Pap）促进与宿主上皮细胞的结合以及随后的定植。菌毛可以在37°C下产生，而这些菌毛的表达在23°C下受到抑制。以前，显示出 rimJ 的插入突变，该基因编码核糖体蛋白S5的N端乙酰转移酶，破坏了这种温度调节反应，从而允许 papBA 在低温下转录。在这项研究中，我们创建了 rimJ 的帧内删除。这种删除不仅减轻了低温的抑制作用，还减轻了丰富的（Luria-Bertani [LB]）培养基和葡萄糖对 papBA 转录的抑制作用，表明RimJ调节 papBA 转录。应对多种环境刺激。还显示 papI 转录受RimJ调控。 papBA 转录也受相变机制控制。我们证明， rimJ 中的 papBA 转录仍需要建立相ON状态所需的调节剂PapI，PapB，Dam，Lrp和环状AMP-CAP。突变株。 rimJ 突变增加了细菌转变为ON相的速率，这表明RimJ抑制了OFF→ON相的转变。 Δ rimJ hns651 突变体在LB培养基上是可行的，但在基本培养基上却不可行。这种合成杀伤力以及转录分析表明，RimJ和H-NS通过不同的途径控制 papBA 转录。 rimJ 中的突变不会极大地影响 fan ， daa 或 fim 操纵子的转录，这表明RimJ可能是 pap 特定的调节器。在 papBA 转录抑制条件下 rimJ 的过表达补充了Δ rimJ 突变，但在激活条件下对转录的影响很小，这表明RimJ调节转录受环境控制。

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