...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Phylogenetic Analysis Reveals Multiple Lateral Transfers of Adenosine-5′-Phosphosulfate Reductase Genes among Sulfate-Reducing Microorganisms
【24h】

Phylogenetic Analysis Reveals Multiple Lateral Transfers of Adenosine-5′-Phosphosulfate Reductase Genes among Sulfate-Reducing Microorganisms

机译：系统发生分析揭示了减少硫酸盐的微生物中腺苷5'-磷酸硫酸还原酶基因的多个横向转移。

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Lateral gene transfer affects the evolutionary path of key genes involved in ancient metabolic traits, such as sulfate respiration, even more than previously expected. In this study, the phylogeny of the adenosine-5′-phosphosulfate (APS) reductase was analyzed. APS reductase is a key enzyme in sulfate respiration present in all sulfate-respiring prokaryotes. A newly developed PCR assay was used to amplify and sequence a fragment (～900 bp) of the APS reductase gene, apsA, from a taxonomically wide range of sulfate-reducing prokaryotes (n = 60). Comparative phylogenetic analysis of all obtained and available ApsA sequences indicated a high degree of sequence conservation in the region analyzed. However, a comparison of ApsA- and 16S rRNA-based phylogenetic trees revealed topological incongruences affecting seven members of the Syntrophobacteraceae and three members of the Nitrospinaceae, which were clearly monophyletic with gram-positive sulfate-reducing bacteria (SRB). In addition, Thermodesulfovibrio islandicus and Thermodesulfobacterium thermophilum, Thermodesulfobacterium commune, and Thermodesulfobacterium hveragerdense clearly branched off between the radiation of the δ-proteobacterial gram-negative SRB and the gram-positive SRB and not close to the root of the tree as expected from 16S rRNA phylogeny. The most parsimonious explanation for these discrepancies in tree topologies is lateral transfer of apsA genes across bacterial divisions. Similar patterns of insertions and deletions in ApsA sequences of donor and recipient lineages provide additional evidence for lateral gene transfer. From a subset of reference strains (n = 25), a fragment of the dissimilatory sulfite reductase genes (dsrAB), which have recently been proposed to have undergone multiple lateral gene transfers (M. Klein et al., J. Bacteriol. 183:6028–6035, 2001), was also amplified and sequenced. Phylogenetic comparison of DsrAB- and ApsA-based trees suggests a frequent involvement of gram-positive and thermophilic SRB in lateral gene transfer events among SRB.
机译：横向基因转移影响涉及古代代谢性状（如硫酸盐呼吸）的关键基因的进化途径，甚至比以前预期的要多。在这项研究中，分析了5'-磷酸腺苷（APS）还原酶的系统发育。 APS还原酶是所有呼吸硫酸盐的原核生物中硫酸盐呼吸的关键酶。一种新开发的PCR分析方法用于从分类广泛的硫酸盐还原原核生物（ n ）中扩增和测序APS还原酶基因 apsA 的片段（〜900 bp）。 em> = 60）。所有获得的和可用的ApsA序列的比较系统发生分析表明，在所分析的区域中高度的序列保守性。但是，对基于ApsA和16S rRNA的系统树的比较显示，拓扑异质性影响了滑膜细菌科的七个成员和旋棘科的三个成员，它们与克明显是一元的。硫酸盐还原菌（SRB）。此外， Thermodesulfovibrio islandicus 和 Thermodesulfobacterium thermophilum ， Thermodesulfobacterium commune 和 Thermodesulfobacterium hveragerdense 显然在辐射之间分叉。 δ细菌革兰氏阴性SRB和革兰氏阳性SRB，并且与16S rRNA系统发育所期望的不接近树的根。对于这些树形差异的最简约的解释是 apsA 基因在细菌分裂中的横向转移。供体和受体谱系的ApsA序列中相似的插入和缺失模式为横向基因转移提供了额外的证据。从参考菌株的一个子集（ n = 25）中，最近提出了异化亚硫酸盐还原酶基因（ dsrAB ）的片段，该片段经历了多次侧向基因转移（M. Klein et al。，J. Bacteriol。183：6028-6035，2001），也得到了扩增和测序。基于DsrAB和ApsA的树的系统发育比较表明，革兰氏阳性和嗜热性SRB经常参与SRB之间的侧向基因转移事件。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号