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首页> 外文期刊>Journal of bacteriology >cis Elements Necessary for Developmental Expression of a Myxococcus xanthus Gene That Depends on C Signaling
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cis Elements Necessary for Developmental Expression of a Myxococcus xanthus Gene That Depends on C Signaling

机译：依赖于C信号的xythus粘球菌基因的发展表达所需的顺式元件

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Cell contact-mediated C signaling coordinates morphogenesis and gene expression during development of Myxococcus xanthus. One promoter that depends on C signaling for transcription lies upstream of Ω4403, the site of a Tn5 lac insertion in the genome. The Ω4403 promoter has a C-box sequence centered at ?49 bp that matches the consensus 5′-CAYYCCY-3′, which is found in several C-signal-dependent promoters. Mutational analysis of the Ω4403 promoter region was performed to test the importance of the C box and to identify other cis-acting elements. A 6-bp change in the ?10 region eliminated promoter activity, but a 6-bp change in the ?35 region decreased activity only about twofold. Certain single-base-pair changes in the C box centered at ?49 bp abolished promoter activity, establishing the importance of this sequence element. Single-base-pair changes in a C-box-like sequence centered at ?77 bp also abolished promoter activity, but the pattern of mutational effects was different from that for the C box centered at ?49 bp. Additional single-base-pair changes indicated that all 10 bp from ?79 to ?70 bp are important for Ω4403 promoter activity. Mutations at ?59, ?61, ?62, and ?63 bp also abolished promoter activity, defining a 5-bp element from ?63 to ?59 bp. This 5-bp element is separated from the 10-bp element (i.e., ?79 to ?70 bp) by 6 bp that can be changed without loss of promoter activity. Likewise, the 5 bp between the 5-bp element and the C box can be changed without loss of activity, but deletion of these 5 bp abolished activity, indicating that spacing is important. Sequences similar to the 5- and 10-bp elements, as well as the C box, are present in other C-signal-dependent promoters, suggesting some similarity in the regulatory mechanisms, but there are also indications that these cis elements do not function identically in the different promoters.
机译：细胞接触介导的C信号传导协调黄连球菌发育过程中的形态发生和基因表达。一个依赖于C信号转录的启动子位于Ω4403的上游，Ω4403是基因组中Tn 5 lac 插入的位点。 Ω4403启动子的C-box序列中心位于〜49 bp，与共有的5'-CAYYCCY-3'相匹配，该序列在几个C信号依赖性启动子中均发现。进行了Ω4403启动子区域的突变分析，以测试C盒的重要性并鉴定其他 cis 作用元件。 β10区域中的6bp变化消除了启动子活性，但是β35区域中的6bp变化降低了活性约两倍。 C盒中某些单一碱基对的变化集中在？49 bp处，从而消除了启动子活性，确立了该序列元件的重要性。 C盒样序列中心位于？77 bp处的单碱基对变化也消除了启动子活性，但突变效应的模式与C盒中心序列位于？49 bp处的模式不同。其他单碱基对变化表明，从？79到？70 bp的所有10 bp对Ω4403启动子活性都很重要。在？59，？61，？62和？63 bp处的突变也消除了启动子活性，从而定义了从63到59 bp的5 bp的元件。该5-bp的元件与10-bp的元件（即〜79至〜70bp）相距6bp，可以改变而不会丢失启动子活性。同样，可以更改5 bp元素和C框之间的5 bp，而不会损失活性，但是删除这5 bp则取消了活性，这表明间隔很重要。其他C信号依赖性启动子中也存在与5和10 bp元件以及C框相似的序列，这提示其调控机制有些相似，但也有迹象表明这些 cis < / em>元素在不同的启动子中的功能不同。

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