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首页> 外文期刊>Journal of bacteriology >The Completely Sequenced Plasmid pEST4011 Contains a Novel IncP1 Backbone and a Catabolic Transposon Harboring tfd Genes for 2,4-Dichlorophenoxyacetic Acid Degradation
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The Completely Sequenced Plasmid pEST4011 Contains a Novel IncP1 Backbone and a Catabolic Transposon Harboring tfd Genes for 2,4-Dichlorophenoxyacetic Acid Degradation

机译：完全测序的质粒pEST4011包含一个新型的IncP1主链和一个带有降解2,4-二氯苯氧基乙酸tfd基因的分解代谢转座子

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The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading bacterium Achromobacter xylosoxidans subsp. denitrificans strain EST4002 contains plasmid pEST4011. This plasmid ensures its host a stable 2,4-D+ phenotype. We determined the complete 76,958-bp nucleotide sequence of pEST4011. This plasmid is a deletion and duplication derivative of pD2M4, the 95-kb highly unstable laboratory ancestor of pEST4011, and was self-generated during different laboratory manipulations performed to increase the stability of the 2,4-D+ phenotype of the original strain, strain D2M4(pD2M4). The 47,935-bp catabolic region of pEST4011 forms a transposon-like structure with identical copies of the hybrid insertion element IS1071::IS1471 at the two ends. The catabolic regions of pEST4011 and pJP4, the best-studied 2,4-D-degradative plasmid, both contain homologous, tfd-like genes for complete 2,4-D degradation, but they have little sequence similarity other than that. The backbone genes of pEST4011 are most similar to the corresponding genes of broad-host-range self-transmissible IncP1 plasmids. The backbones of the other three IncP1 catabolic plasmids that have been sequenced (the 2,4-D-degradative plasmid pJP4, the haloacetate-catabolic plasmid pUO1, and the atrazine-catabolic plasmid pADP-1) are nearly identical to the backbone of R751, the archetype plasmid of the IncP1 β subgroup. We show that despite the overall similarity in plasmid organization, the pEST4011 backbone is sufficiently different (51 to 86% amino acid sequence identity between individual backbone genes) from the backbones of members of the three IncP1 subgroups (theα , β, and γ subgroups) that it belongs to a new IncP1subgroup, the δ subgroup. This conclusion was also supported by a phylogenetic analysis of the trfA2, korA, and traG gene products of different IncP1 plasmids.
机译：降解除草剂2,4-二氯苯氧基乙酸（2,4-D）的细菌木氧化无色杆菌亚种。 denitrificans 菌株EST4002含有质粒pEST4011。该质粒确保其宿主具有稳定的2,4-D + 表型。我们确定了pEST4011的完整的76,958-bp核苷酸序列。该质粒是pEST4011的95 kb高度不稳定实验室祖先pD2M4的缺失和重复衍生物，在为提高2,4-D + 原始菌株D2M4（pD2M4）的表型。 pEST4011的47,935-bp分解代谢区域形成了转座子样结构，在两端具有相同副本的杂种插入元件IS 1071 :: IS 1471 。研究最深入的2,4-D降解质粒pEST4011和pJP4的分解代谢区域均含有同源的 tfd 样基因，可完全降解2,4-D，但序列很少除此之外。 pEST4011的主链基因与广泛宿主范围自我传播的IncP1质粒的相应基因最为相似。已测序的其他三个IncP1分解代谢质粒的骨架（2,4-D降解质粒pJP4，卤代乙酸酯分解质粒pUO1和and去津分解代谢质粒pADP-1）与R751的骨架几乎相同，IncP1β亚型的原型质粒。我们显示，尽管质粒组织总体上相似，但pEST4011主链与三个IncP1子组（α，β和γ子组）的成员的主链充分不同（单个主链基因之间51至86％的氨基酸序列同一性）它属于新的IncP1子组δ子组。对不同IncP1质粒的 trfA2 ， korA 和 traG 基因产物的系统发育分析也支持该结论。

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