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首页> 外文期刊>Journal of bacteriology >The Level of AdpA Directly Affects Expression of Developmental Genes in Streptomyces coelicolor
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The Level of AdpA Directly Affects Expression of Developmental Genes in Streptomyces coelicolor

机译：AdpA水平直接影响天蓝色链霉菌中发育基因的表达。

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摘要

AdpA is a key regulator of morphological differentiation in Streptomyces. In contrast to Streptomyces griseus, relatively little is known about AdpA protein functions in Streptomyces coelicolor. Here, we report for the first time the translation accumulation profile of the S. coelicolor adpA (adpASc) gene; the level of S. coelicolor AdpA (AdpASc) increased, reaching a maximum in the early stage of aerial mycelium formation (after 36 h), and remained relatively stable for the next several hours (48 to 60 h), and then the signal intensity decreased considerably. AdpASc specifically binds the adpASc promoter region in vitro and in vivo, suggesting that its expression is autoregulated; surprisingly, in contrast to S. griseus, the protein presumably acts as a transcriptional activator. We also demonstrate a direct influence of AdpASc on the expression of several genes whose products play key roles in the differentiation of S. coelicolor: STI, a protease inhibitor; RamR, an atypical response regulator that itself activates expression of the genes for a small modified peptide that is required for aerial growth; and ClpP1, an ATP-dependent protease. The diverse influence of AdpASc protein on the expression of the analyzed genes presumably results mainly from different affinities of AdpASc protein to individual promoters.
机译：AdpA是链霉菌中形态分化的关键调节剂。与灰色链霉菌相比，关于天蓝色链霉菌中AdpA蛋白功能的了解相对较少。在这里，我们首次报告天蓝色链霉菌 adpA （ adpA Sc ）基因的翻译积累概况;肠球菌S. coelicolor AdpA（AdpA Sc ）的水平升高，在气生菌丝体形成的早期阶段（36小时后）达到最大值，并在相对稳定的时间内保持稳定。接下来的几个小时（48至60小时），然后信号强度显着下降。 AdpA Sc 特异性结合 adpA Sc 启动子区域体外和在体内，表明其表达是自动调节的;令人惊讶地，与灰链霉菌相比，该蛋白可能起转录激活剂的作用。我们还证明了AdpA Sc 对几种基因表达的直接影响，这些基因的产物在大肠杆菌的分离中起关键作用。 RamR，一种非典型应答调节剂，其自身激活气生生长所需的小的修饰肽的基因表达; ClpP1，一种ATP依赖性蛋白酶。 AdpA Sc 蛋白对分析基因表达的不同影响可能主要是由于AdpA Sc 单个启动子的蛋白质。

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