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首页> 外文期刊>Journal of bacteriology >The trpE Gene Negatively Regulates Differentiation of Heterocysts at the Level of Induction in Anabaena sp. Strain PCC 7120
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The trpE Gene Negatively Regulates Differentiation of Heterocysts at the Level of Induction in Anabaena sp. Strain PCC 7120

机译：trpE基因在鱼腥藻的诱导水平上负调控着胚泡的分化。应变PCC 7120

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摘要

Levels of 2-oxoglutarate (2-OG) reflect nitrogen status in many bacteria. In heterocystous cyanobacteria, a spike in the 2-OG level occurs shortly after the removal of combined nitrogen from cultures and is an integral part of the induction of heterocyst differentiation. In this work, deletion of one of the two annotated trpE genes in Anabaena sp. strain PCC 7120 resulted in a spike in the 2-OG level and subsequent differentiation of a wild-type pattern of heterocysts when filaments of the mutant were transferred from growth on ammonia to growth on nitrate. In contrast, 2-OG levels were unaffected in the wild type, which did not differentiate under the same conditions. An inverted-repeat sequence located upstream of trpE bound a central regulator of differentiation, HetR, in vitro and was necessary for HetR-dependent transcription of a reporter fusion and complementation of the mutant phenotype in vivo. Functional complementation of the mutant phenotype with the addition of tryptophan suggested that levels of tryptophan, rather than the demonstrated anthranilate synthase activity of TrpE, mediated the developmental response of the wild type to nitrate. A model is presented for the observed increase in 2-OG in the trpE mutant.
机译：2-氧戊二酸（2-OG）的水平反映了许多细菌中的氮状态。在异性蓝藻细菌中，从培养物中除去合并的氮后不久，2-OG浓度就会出现峰值，这是诱导异性藻细胞分化的重要组成部分。在这项工作中，删除了Anabaena sp。中两个带注释的 trpE 基因之一。当突变体的细丝从在氨上的生长转移到在硝酸盐上的生长时，PCC 7120菌株导致2-OG水平升高，并随后分化出了异型囊肿的野生型。相反，在野生型中2-OG水平不受影响，在相同条件下没有区别。位于 trpE 上游的反向重复序列结合了分化的中心调控因子HetR，在体外，对于报道融合和互补的HetR依赖性转录是必需的。体内 的突变表型。添加色氨酸的突变表型的功能互补表明色氨酸的水平而不是已证明的TrpE的邻氨基苯甲酸酯合酶活性介导了野生型对硝酸盐的发育反应。提出了一个模型，用于观察 trpE 突变体中2-OG的增加。

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