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首页> 外文期刊>Journal of bacteriology >Characterization of the ArsRS Regulon of Helicobacter pylori, Involved in Acid Adaptation
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Characterization of the ArsRS Regulon of Helicobacter pylori, Involved in Acid Adaptation

机译：幽门螺杆菌的ArsRS调节子的表征，涉及酸适应

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The human gastric pathogen Helicobacter pylori is extremely well adapted to the highly acidic conditions encountered in the stomach. The pronounced acid resistance of H. pylori relies mainly on the ammonia-producing enzyme urease; however, urease-independent mechanisms are likely to contribute to acid adaptation. Acid-responsive gene regulation is mediated at least in part by the ArsRS two-component system consisting of the essential OmpR-like response regulator ArsR and the nonessential cognate histidine kinase ArsS, whose autophosphorylation is triggered in response to low pH. In this study, by global transcriptional profiling of an ArsS-deficient H. pylori mutant grown at pH 5.0, we define the ArsR～P-dependent regulon consisting of 109 genes, including the urease gene cluster, the genes encoding the aliphatic amidases AmiE and AmiF, and the rocF gene encoding arginase. We show that ArsR～P controls the acid-induced transcription of amiE and amiF by binding to extended regions located upstream of the ?10 box of the respective promoters. In contrast, transcription of rocF is repressed by ArsR～P at neutral, acidic, and mildly alkaline pH via high-affinity binding of the response regulator to a site overlapping the promoter of the rocF gene.
机译：人胃病原体幽门螺杆菌非常适合胃中遇到的高酸性条件。 H的明显耐酸性。幽门螺旋杆菌主要依靠产氨酶脲酶。然而，不依赖脲酶的机制可能有助于酸适应。酸响应基因的调节至少部分由ArsRS两组分系统介导，该系统由必需的OmpR样响应调节剂ArsR和非必需同源组氨酸激酶ArsS组成，后者响应低pH值会触发自身磷酸化。在这项研究中，通过对ArsS缺陷的 H进行全局转录分析。 pylori 突变体在pH 5.0下生长，我们定义了由109个基因组成的ArsR〜P依赖的调控子，包括脲酶基因簇，编码脂肪族酰胺酶AmiE和AmiF的基因以及 rocF 编码精氨酸酶的基因。我们发现，ArsR〜P通过结合到位于各个启动子的？10框上游的延伸区域来控制 amiE 和 amiF 的酸诱导的转录。相反，在中性，酸性和弱碱性pH值下，ArsR〜P通过应答调节剂与 rocF <启动子重叠的位点的高亲和力结合，抑制了 rocF 的转录。 / em>基因。

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