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首页> 外文期刊>Journal of bacteriology >Differential Proteomic Analysis of the Bacillus anthracis Secretome: Distinct Plasmid and Chromosome CO2-Dependent Cross Talk Mechanisms Modulate Extracellular Proteolytic Activities
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Differential Proteomic Analysis of the Bacillus anthracis Secretome: Distinct Plasmid and Chromosome CO2-Dependent Cross Talk Mechanisms Modulate Extracellular Proteolytic Activities

机译：炭疽芽孢杆菌分泌蛋白质组的蛋白质组学分析：质粒和染色体依赖CO2的相声机制调节细胞外蛋白水解活性。

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The secretomes of a virulent Bacillus anthracis strain and of avirulent strains (cured of the virulence plasmids pXO1 and pXO2), cultured in rich and minimal media, were studied by a comparative proteomic approach. More than 400 protein spots, representing the products of 64 genes, were identified, and a unique pattern of protein relative abundance with respect to the presence of the virulence plasmids was revealed. In minimal medium under high CO2 tension, conditions considered to simulate those encountered in the host, the presence of the plasmids leads to enhanced expression of 12 chromosome-carried genes (10 of which could not be detected in the absence of the plasmids) in addition to expression of 5 pXO1-encoded proteins. Furthermore, under these conditions, the presence of the pXO1 and pXO2 plasmids leads to the repression of 14 chromosomal genes. On the other hand, in minimal aerobic medium not supplemented with CO2, the virulent and avirulent B. anthracis strains manifest very similar protein signatures, and most strikingly, two proteins (the metalloproteases InhA1 and NprB, orthologs of gene products attributed to the Bacillus cereus group PlcR regulon) represent over 90% of the total secretome. Interestingly, of the 64 identified gene products, at least 31 harbor features characteristic of virulence determinants (such as toxins, proteases, nucleotidases, sulfatases, transporters, and detoxification factors), 22 of which are differentially regulated in a plasmid-dependent manner. The nature and the expression patterns of proteins in the various secretomes suggest that distinct CO2-responsive chromosome- and plasmid-encoded regulatory factors modulate the secretion of potential novel virulence factors, most of which are associated with extracellular proteolytic activities.
机译：通过比较蛋白质组学的方法研究了在丰富的培养基和最少的培养基中培养的有毒的炭疽芽孢杆菌菌株和无毒菌株（由毒力质粒pXO1和pXO2固化）的分泌组。鉴定出代表64个基因的产物的400多个蛋白斑点，并揭示了关于毒力质粒存在的蛋白质相对丰度的独特模式。在高CO 2 张力下的最小培养基中，该条件被认为可以模拟宿主中遇到的那些条件，质粒的存在导致12个染色体携带基因的表达增强（其中10个在染色体中无法检测到）。除了表达5种pXO1编码的蛋白质外，还包括质粒的缺失。此外，在这些条件下，pXO1和pXO2质粒的存在导致14个染色体基因的阻遏。另一方面，在不添加CO 2 的基本需氧培养基中，强毒和无毒的 B。炭疽菌菌株表现出非常相似的蛋白质特征，最引人注目的是，两种蛋白质（金属蛋白酶InhA1和NprB，蜡状芽胞杆菌基因组直向同源物归因于蜡状芽胞杆菌PlcR调节子）占90％以上。总分泌组。有趣的是，在64种已鉴定的基因产物中，至少有31个具有毒力决定簇（例如毒素，蛋白酶，核苷酸酶，硫酸酯酶，转运蛋白和解毒因子）特征的港口特征，其中22个以质粒依赖性方式被差异调节。各种分泌蛋白中蛋白质的性质和表达模式表明，不同的CO 2 反应性染色体和质粒编码调控因子调节潜在的新型毒力因子的分泌，其中大多数与细胞外有关蛋白水解活性。

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