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首页> 外文期刊>Journal of bacteriology >Flavonoid-Inducible Modifications to Rhamnan O Antigens Are Necessary for Rhizobium sp. Strain NGR234-Legume Symbioses
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Flavonoid-Inducible Modifications to Rhamnan O Antigens Are Necessary for Rhizobium sp. Strain NGR234-Legume Symbioses

机译：类黄酮诱导抗原对Rhamnan O抗原的类黄酮诱导的修饰。 NGR234-豆类共生菌株

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Rhizobium sp. strain NGR234 produces a flavonoid-inducible rhamnose-rich lipopolysaccharide (LPS) that is important for the nodulation of legumes. Many of the genes encoding the rhamnan part of the molecule lie between 87° and 110° of pNGR234a, the symbiotic plasmid of NGR234. Computational methods suggest that 5 of the 12 open reading frames (ORFs) within this arc are involved in synthesis (and subsequent polymerization) of l-rhamnose. Two others probably play roles in the transport of carbohydrates. To evaluate the function of these ORFs, we mutated a number of them and tested the ability of the mutants to nodulate a variety of legumes. At the same time, changes in the production of surface polysaccharides (particularly the rhamnan O antigen) were examined. Deletion of rmlB to wbgA and mutation in fixF abolished rhamnan synthesis. Mutation of y4gM (a member of the ATP-binding cassette transporter family) did not abolish production of the rhamnose-rich LPS but, unexpectedly, the mutant displayed a symbiotic phenotype very similar to that of strains unable to produce the rhamnan O antigen (NGRΔrmlB-wbgA and NGRΩfixF). At least two flavonoid-inducible regulatory pathways are involved in synthesis of the rhamnan O antigen. Mutation of either pathway reduces rhamnan production. Coordination of rhamnan synthesis with rhizobial release from infection threads is thus part of the symbiotic interaction.
机译：根瘤菌 sp。 NGR234菌株可产生类黄酮诱导的富含鼠李糖的脂多糖（LPS），这对于豆科植物的结节很重要。 NGR234的共生质粒pNGR234 a 的许多编码该分子的鼠李糖苷部分的基因位于87°至110°之间。计算方法表明，该弧内的12个开放阅读框（ORF）中有5个参与了1-鼠李糖的合成（和随后的聚合）。另外两个可能在碳水化合物的运输中起作用。为了评估这些ORF的功能，我们突变了许多突变体并测试了突变体结瘤各种豆类的能力。同时，检查了表面多糖（特别是鼠李糖O型抗原）的生产变化。将 rmlB 删除为 wbgA 和 fixF 中的突变取消了鼠李糖聚糖的合成。 y4gM（ATP结合盒转运蛋白家族的成员）的突变并未消除鼠李糖丰富的LPS的产生，但出乎意料的是，该突变体表现出与无法产生鼠李糖蛋白O抗原（NGRΔ）的菌株非常相似的共生表型。 rmlB-wbgA 和NGRΩ fixF ）。鼠李糖聚糖O抗原的合成涉及至少两种类黄酮诱导的调节途径。任一途径的突变都会降低鼠李糖聚糖的产量。因此，鼠李糖聚糖合成与从感染丝中释放的根瘤菌的协调是共生相互作用的一部分。

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