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首页> 外文期刊>Journal of bacteriology >Localization of the Naturally Occurring Plasmid ColE1 at the Cell Pole
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Localization of the Naturally Occurring Plasmid ColE1 at the Cell Pole

机译：自然出现的质粒ColE1在细胞极的定位

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The naturally occurring plasmid ColE1 was found to localize as a cluster in one or both of the cell poles of Escherichia coli. In addition to the polar localization of ColE1 in most cells, movement of the plasmid to the midcell position was observed in time-lapse studies. ColE1 could be displaced from its polar location by the p15A replicon, pBAD33, but not by plasmid RK2. The displacement of ColE1 by pBAD33 resulted in an almost random positioning of ColE1 foci in the cell and also in a loss of segregational stability, as evidenced by the large number of cells carrying pBAD33 with no visible ColE1 focus and as confirmed by ColE1 stability studies. The addition of the active partitioning systems of the F plasmid (sopABC) or RK2 (OB1 incC korB) resulted in movement of the ColE1 replicon from the cell pole to within the nucleoid region. This repositioning did not result in destabilization but did result in an increase in the number of plasmid foci, most likely due to partial declustering. These results are consistent with the importance of par regions to the localization of plasmids to specific regions of the cell and demonstrate both localization and dynamic movement for a naturally occurring plasmid that does not encode a replication initiation protein or a partitioning system that is required for plasmid stability.
机译：发现天然存在的质粒ColE1以簇的形式位于大肠杆菌的一个或两个细胞极中。除了ColE1在大多数细胞中的极性定位外，在延时研究中还观察到质粒向中细胞位置移动。 ColE1可以通过p15A复制子pBAD33从其极性位置转移，但不能通过质粒RK2转移。 pBAD33对ColE1的置换导致ColE1病灶在细胞中几乎随机定位，并且也导致了分离稳定性的丧失，这由大量携带pBAD33且没有可见ColE1焦点的细胞所证实，并已由ColE1稳定性研究证实。 F质粒（ sopABC ）或RK2（O B1 incC korB ）的活性分配系统的加入导致ColE1复制子的运动从细胞极到核苷区域内。这种重新定位不会导致不稳定，但确实会导致质粒病灶数量增加，这很可能是由于部分解簇。这些结果与 par 区域对质粒定位到细胞特定区域的重要性相一致，并证明了天然存在的不编码复制起始蛋白或蛋白的质粒的定位和动态移动质粒稳定性所需的分配系统。

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