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首页> 外文期刊>Journal of bacteriology >Proteomic Characterization of the Rhodobacter sphaeroides 2.4.1 Photosynthetic Membrane: Identification of New Proteins
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Proteomic Characterization of the Rhodobacter sphaeroides 2.4.1 Photosynthetic Membrane: Identification of New Proteins

机译：球形红球菌2.4.1光合膜的蛋白质组学表征：新蛋白的鉴定。

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The Rhodobacter sphaeroides intracytoplasmic membrane (ICM) is an inducible membrane that is dedicated to the major events of bacterial photosynthesis, including harvesting light energy, separating primary charges, and transporting electrons. In this study, multichromatographic methods coupled with Fourier transform ion cyclotron resonance mass spectrometry, combined with subcellular fractionation, was used to test the hypothesis that the photosynthetic membrane of R. sphaeroides 2.4.1 contains a significant number of heretofore unidentified proteins in addition to the integral membrane pigment-protein complexes, including light-harvesting complexes 1 and 2, the photochemical reaction center, and the cytochrome bc1 complex described previously. Purified ICM vesicles are shown to be enriched in several abundant, newly identified membrane proteins, including a protein of unknown function (AffyChip designation RSP1760) and a possible alkane hydroxylase (RSP1467). When the genes encoding these proteins are mutated, specific photosynthetic phenotypes are noted, illustrating the potential new insights into solar energy utilization to be gained by this proteomic blueprint of the ICM. In addition, proteins necessary for other cellular functions, such as ATP synthesis, respiration, solute transport, protein translocation, and other physiological processes, were also identified to be in association with the ICM. This study is the first to provide a more global view of the protein composition of a photosynthetic membrane from any source. This protein blueprint also provides insights into potential mechanisms for the assembly of the pigment-protein complexes of the photosynthetic apparatus, the formation of the lipid bilayer that houses these integral membrane proteins, and the possible functional interactions of ICM proteins with activities that reside in domains outside this specialized bioenergetic membrane.
机译：球形红球菌胞质内膜（ICM）是一种可诱导膜，专门用于细菌光合作用的主要事件，包括收获光能，分离初级电荷和传输电子。在这项研究中，多色谱方法与傅里叶变换离子回旋共振质谱联用，结合亚细胞分馏，被用于检验关于 R的光合膜的假设。 sphaeroides 2.4.1除完整的膜色素-蛋白质复合物外，还包含大量迄今尚未鉴定的蛋白质，包括光捕获复合物1和2，光化学反应中心和细胞色素 bc 1 复合体。纯化的ICM囊泡富含一些新近鉴定的丰富膜蛋白，包括功能未知的蛋白（AffyChip名称为RSP1760）和可能的烷烃羟化酶（RSP1467）。当编码这些蛋白质的基因发生突变时，就会注意到特定的光合作用表型，这说明了通过ICM的蛋白质组学蓝图可以获得对太阳能利用的潜在新见解。此外，还确定了其他细胞功能（如ATP合成，呼吸作用，溶质转运，蛋白质易位和其他生理过程）必需的蛋白质与ICM相关。这项研究是第一个提供来自任何来源的光合膜蛋白质组成更全面视图的研究。该蛋白质蓝图还提供了有关光合作用色素-蛋白质复合物组装的潜在机制，容纳这些完整膜蛋白的脂质双层形成以及ICM蛋白与结构域中活性之间可能的功能相互作用的潜在机制的见解。在这种专门的生物能膜之外。

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