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首页> 外文期刊>Journal of bacteriology >Induction of RpoS Degradation by the Two-Component System Regulator RstA in Salmonella enterica
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Induction of RpoS Degradation by the Two-Component System Regulator RstA in Salmonella enterica

机译：两组分系统调节剂RstA在肠沙门氏菌中诱导RpoS降解

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Bacterial survival in diverse and changing environments relies on the accurate interplay between different regulatory pathways, which determine the design of an adequate adaptive response. The proper outcome depends on a precise gene expression profile generated from the finely tuned and concerted action of transcriptional factors of distinct regulatory hierarchies. Salmonella enterica serovar Typhimurium harbors multiple regulatory systems that are crucial for the bacterium to cope with harsh extra- and intracellular environments. In this work, we found that the expression of Salmonella RstA, a response regulator from the two-component system family, was able to downregulate the expression of three RpoS-controlled genes (narZ, spvA, and bapA). Furthermore, this downregulation was achieved by a reduction in RpoS cellular levels. The alternative sigma factor RpoS is critical for bacterial endurance under the most-stressful conditions, including stationary-phase entrance and host adaptation. Accordingly, RpoS cellular levels are tightly controlled by complex transcriptional, translational, and posttranslational mechanisms. The analysis of each regulatory step revealed that in Salmonella, RstA expression was able to promote RpoS degradation independently of the MviA-ClpXP proteolytic pathway. Additionally, we show that RstA is involved in modulating Salmonella biofilm formation. The fact that the RpoS-modulated genes affected by RstA expression have previously been demonstrated to contribute to Salmonella pathogenic traits, which include biofilm-forming capacity, suggests that under yet unknown conditions, RstA may function as a control point of RpoS-dependent pathways that govern Salmonella virulence.
机译：细菌在各种不断变化的环境中的生存依赖于不同调节途径之间的准确相互作用，这决定了适当的适应性反应的设计。正确的结果取决于精确的基因表达谱，该谱是由不同调控层次的转录因子的精细调节和协同作用产生的。鼠伤寒沙门氏菌具有多种调节系统，这些系统对于细菌应对恶劣的细胞外和细胞内环境至关重要。在这项工作中，我们发现来自两个组件系统家族的应答调节物 Salmonella RstA的表达能够下调三个RpoS调控基因（ narZ ， spvA 和 bapA ）。此外，这种下调是通过降低RpoS细胞水平来实现的。替代的Sigma因子RpoS对于最紧张条件下的细菌耐力至关重要，包括固定相进入和宿主适应。因此，RpoS细胞水平受到复杂的转录，翻译和翻译后机制的严格控制。对每个调节步骤的分析表明，在沙门氏菌中，RstA表达能够独立于MviA-ClpXP蛋白水解途径而促进RpoS降解。此外，我们表明RstA参与调节沙门氏菌生物膜形成。先前已证明受RstA表达影响的RpoS调节基因可导致沙门氏菌致病性状，包括生物膜形成能力，这一事实表明，在未知条件下，RstA可能作为对照。沙门氏菌毒力的RpoS依赖途径的关键点。

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