...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Human- and Plant-Pathogenic Pseudomonas Species Produce Bacteriocins Exhibiting Colicin M-Like Hydrolase Activity towards Peptidoglycan Precursors
【24h】

Human- and Plant-Pathogenic Pseudomonas Species Produce Bacteriocins Exhibiting Colicin M-Like Hydrolase Activity towards Peptidoglycan Precursors

机译：人类和植物致病性假单胞菌种产生对肽聚糖前体表现出Colicin M-like水解酶活性的细菌素。

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Genes encoding proteins that exhibit similarity to the C-terminal domain of Escherichia coli colicin M were identified in the genomes of some Pseudomonas species, namely, P. aeruginosa, P. syringae, and P. fluorescens. These genes were detected only in a restricted number of strains. In P. aeruginosa, for instance, the colicin M homologue gene was located within the ExoU-containing genomic island A, a large horizontally acquired genetic element and virulence determinant. Here we report the cloning of these genes from the three Pseudomonas species and the purification and biochemical characterization of the different colicin M homologues. All of them were shown to exhibit Mg2+-dependent diphosphoric diester hydrolase activity toward the two undecaprenyl phosphate-linked peptidoglycan precursors (lipids I and II) in vitro. In all cases, the site of cleavage was localized between the undecaprenyl and pyrophospho-MurNAc moieties of these precursors. These enzymes were not active on the cytoplasmic precursor UDP-MurNAc-pentapeptide or (or only very poorly) on undecaprenyl pyrophosphate. These colicin M homologues have a narrow range of antibacterial activity. The P. aeruginosa protein at low concentrations was shown to inhibit growth of sensitive P. aeruginosa strains. These proteins thus represent a new class of bacteriocins (pyocins), the first ones reported thus far in the genus Pseudomonas that target peptidoglycan metabolism.
机译：在某些 Pseudomonas 物种（ P）的基因组中鉴定出了编码与大肠杆菌 colicin M C末端结构域相似的蛋白质的基因。铜绿假单胞菌，丁香假单胞菌和 P。荧光。仅在有限数量的菌株中检测到这些基因。在 P中。例如，在铜绿假单胞菌中，大肠菌素M同源基因位于含有ExoU的基因组岛A中，该岛是水平获得的大型遗传元件和毒力决定因素。在这里，我们报告了从三个 Pseudomonas 物种克隆这些基因以及不同大肠菌素M同系物的纯化和生化特性。它们都显示出对Mg 2 + 依赖性的二磷酸二酯水解酶活性，对两种十一碳烯基磷酸酯连接的肽聚糖前体（脂质I和II）具有活性。在所有情况下，切割位点都位于这些前体的十一碳烯基和焦磷酸-MurNAc部分之间。这些酶对细胞质前体UDP-MurNAc-五肽或（或非常差）对癸烯酸焦磷酸没有活性。这些大肠菌素M同系物具有窄范围的抗菌活性。 P。低浓度的铜绿蛋白可以抑制敏感的 P的生长。铜绿菌株。因此，这些蛋白质代表了一类新的细菌素（脓菌素），到目前为止，是在 Pseudomonas 属中主要针对肽聚糖代谢的蛋白质。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号