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首页> 外文期刊>Journal of bacteriology >Identification and Characterization of Hemolysin-Like Proteins Similar to RTX Toxin in Pasteurella pneumotropica
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Identification and Characterization of Hemolysin-Like Proteins Similar to RTX Toxin in Pasteurella pneumotropica

机译：嗜肺巴斯德氏菌中类似于RTX毒素的溶血素类似蛋白的鉴定和表征

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Pasteurella pneumotropica is an opportunistic pathogen that causes lethal pneumonia in immunodeficient rodents. The virulence factors of this bacterium remain unknown. In this study, we identified the genes encoding two RTX toxins, designated as pnxI and pnxII, from the genomic DNA of P. pneumotropica ATCC 35149 and characterized with respect to hemolysis. The pnxI operon was organized according to the manner in which the genes encoded the structural RTX toxin (pnxIA), the type I secretion systems (pnxIB and pnxID), and the unknown orf. The pnxII gene was involved only with the pnxIIA that coded for a structural RTX toxin. Both the structural RTX toxins of deduced PnxIA and PnxIIA were involved in seven of the RTX repeat and repeat-like sequences. By quantitative PCR analysis of the structural RTX toxin-encoding genes in P. pneumotropica ATCC 35149, the gene expression of pnxIA was found to have increased from the early log phase, while that of pnxIIA increased from the late log to the early stationary phase. As expressed in Escherichia coli, both the recombinant proteins of PnxIA and PnxIIA showed weak hemolytic activity in both sheep and murine erythrocytes. On the basis of the results of the Southern blotting analysis, the pnxIA gene was detected in 82% of the isolates, while the pnxIIA gene was detected in 39%. These results indicate that the products of both pnxIA and pnxIIA were putative associations of virulence factors in the rodent pathogen P. pneumotropica.
机译： Pasteurella pneumotropica 是一种机会病原体，可在免疫缺陷的啮齿动物中引起致命的肺炎。该细菌的致病因子仍然未知。在这项研究中，我们从 P的基因组DNA中鉴定了编码两种RTX毒素的基因，分别命名为 pnxI 和 pnxII 。肺气肿 ATCC 35149，具有溶血作用。根据基因编码结构性RTX毒素（ pnxIA ），I型分泌系统（ pnxIB ）的方式来组织 pnxI 操纵子。和 pnxID ），以及未知的 orf 。 pnxII 基因仅与编码结构性RTX毒素的 pnxIIA 有关。推导的PnxIA和PnxIIA的结构性RTX毒素都参与了七个RTX重复序列和重复序列样序列。通过定量PCR分析 P中结构性RTX毒素编码基因。 pneumotropica ATCC 35149，发现 pnxIA 的基因表达从对数早期开始增加，而 pnxIIA 的基因表达从对数晚期到早期增加固定相。如在大肠杆菌中表达的，PnxIA和PnxIIA的重组蛋白在绵羊和鼠的红细胞中均显示出弱的溶血活性。根据Southern印迹分析的结果，在82％的分离物中检测到 pnxIA 基因，而在39％的分离物中检测到 pnxIIA 基因。这些结果表明， pnxIA 和 pnxIIA 的产物都是啮齿动物病原体 P中毒力因子的推定关联。肺炎。

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