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首页> 外文期刊>Journal of bacteriology >Flavobacterium johnsoniae gldN and gldO Are Partially Redundant Genes Required for Gliding Motility and Surface Localization of SprB
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Flavobacterium johnsoniae gldN and gldO Are Partially Redundant Genes Required for Gliding Motility and Surface Localization of SprB

机译：约翰逊黄杆菌gldN和gldO是滑行运动和SprB表面定位所需的部分冗余基因

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Cells of the gliding bacterium Flavobacterium johnsoniae move rapidly over surfaces. Mutations in gldN cause a partial defect in gliding. A novel bacteriophage selection strategy was used to aid construction of a strain with a deletion spanning gldN and the closely related gene gldO in an otherwise wild-type F. johnsoniae UW101 background. Bacteriophage transduction was used to move a gldN mutation into F. johnsoniae UW101 to allow phenotypic comparison with the gldNO deletion mutant. Cells of the gldN mutant formed nonspreading colonies on agar but retained some ability to glide in wet mounts. In contrast, cells of the gldNO deletion mutant were completely nonmotile, indicating that cells require GldN, or the GldN-like protein GldO, to glide. Recent results suggest that Porphyromonas gingivalis PorN, which is similar in sequence to GldN, has a role in protein secretion across the outer membrane. Cells of the F. johnsoniae gldNO deletion mutant were defective in localization of the motility protein SprB to the cell surface, suggesting that GldN may be involved in secretion of components of the motility machinery. Cells of the gldNO deletion mutant were also deficient in chitin utilization and were resistant to infection by bacteriophages, phenotypes that may also be related to defects in protein secretion.
机译：滑行细菌约翰逊黄藻的细胞在表面快速移动。 gldN 中的突变会导致滑行的部分缺陷。一种新颖的噬菌体选择策略被用于辅助构建在其他野生型 F中缺失 gldN 和紧密相关的基因 gldO 的菌株。 johnsoniae UW101背景。噬菌体转导用于将 gldN 突变移入 F。 johnsoniae UW101可以与 gldNO 缺失突变体进行表型比较。 gldN 突变体的细胞在琼脂上形成未扩散的菌落，但保留了在潮湿的山中滑行的能力。相反， gldNO 缺失突变体的细胞完全不活动，表明细胞需要GldN或类似GldN的蛋白GldO才能滑行。最近的结果表明，牙龈卟啉单胞菌 PorN与GldN的序列相似，在整个外膜的蛋白质分泌中起作用。 F的单元格。 johnsoniae gldNO 缺失突变体在运动蛋白SprB定位于细胞表面方面存在缺陷，表明GldN可能参与了运动机能的分泌。 gldNO 缺失突变体的细胞也缺乏几丁质的利用，并且对噬菌体（表型也可能与蛋白质分泌缺陷有关）的感染具有抵抗力。

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