...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Sigma Factor F Does Not Prevent Rifampin Inhibition of RNA Polymerase or Cause Rifampin Tolerance in Mycobacterium tuberculosis
【24h】

Sigma Factor F Does Not Prevent Rifampin Inhibition of RNA Polymerase or Cause Rifampin Tolerance in Mycobacterium tuberculosis

机译：西格玛因子F不会阻止利福平抑制RNA聚合酶或引起结核分枝杆菌对利福平的耐受

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The tolerance of Mycobacterium tuberculosis to antituberculosis drugs is a major reason for the lengthy therapy needed to treat a tuberculosis infection. Rifampin is a potent inhibitor of RNA polymerase (RNAP) in vivo but has been shown to be less effective against stationary-phase bacteria. Sigma factor F is associated with bacteria entering stationary phase and has been proposed to impact rifampin activity. Here we investigate whether RNAP containing SigF is more resistant to rifampin inhibition in vitro and whether overexpression of sigF renders M. tuberculosis more tolerant to rifampin. Real-time and radiometric in vitro transcription assays revealed that rifampin equally inhibits transcription by RNAP containing sigma factors SigA and SigF, therefore ruling out the hypothesis that SigF may be responsible for increased resistance of the enzyme to rifampin in vitro. In addition, overexpression or deletion of sigF did not alter rifampin susceptibility in axenic cultures of M. tuberculosis, indicating that SigF does not affect rifampin tolerance in vivo.
机译：结核分枝杆菌对抗结核药物的耐受性是治疗结核感染所需的长期治疗的主要原因。利福平是体内有效的RNA聚合酶（RNAP）抑制剂，但已证明对固定相细菌无效。西格玛因子F与细菌进入固定相有关，并已被提议影响利福平的活性。在这里，我们研究了含有SigF的RNAP是否在体外对 的利福平抑制作用更有抗性，以及 sigF 的过度表达是否会导致 M。结核病对利福平更具耐受性。实时和体外放射定量分析表明，利福平同样抑制包含sigma因子SigA和SigF的RNAP的转录，因此排除了SigF可能导致酶对利福平抗性增加的假设。 em>体外。此外， sigF 的过表达或缺失不会改变 M的无菌培养物中的利福平敏感性。结核病，表明SigF不会影响体内的利福平耐受性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号