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首页> 外文期刊>Journal of bacteriology >The Fsr Quorum-Sensing System of Enterococcus faecalisModulates Surface Display of the Collagen-Binding MSCRAMM Ace through Regulation of gelE
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The Fsr Quorum-Sensing System of Enterococcus faecalisModulates Surface Display of the Collagen-Binding MSCRAMM Ace through Regulation of gelE

机译：粪肠球菌的Fsr群体感应系统通过调节gelE调节结合胶原的MSCRAMM Ace的表面展示

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Ace, a known virulence factor and the first identified microbial surface component recognizing adhesive matrix molecule (MSCRAMM) of Enterococcus faecalisis associated with host cell adherence and endocarditis. The Fsr quorum-sensing system of E. faecalis, a two-component signal transduction system, has also been repeatedly linked to virulence in E. faecalis, due in part to the transcriptional induction of an extracellular metalloprotease, gelatinase (GelE). In this study, we discovered that disruption of the Fsr pathway significantly increased the levels of Ace on the cell surface in the latter phases of growth. Furthermore, we observed that, in addition to fsrBmutants, other strains identified as deficient in GelE activity also demonstrated a similar phenotype. Additional experiments demonstrated the GelE-dependent cleavage of Ace from the surface of E. faecalis, confirming that GelE specifically reduces Ace cell surface display. In addition, disruption of the Fsr system or GelE expression significantly improved the ability of E. faecalisto adhere to collagen, which is consistent with higher levels of Ace on the E. faecalissurface. These results demonstrate that the display of Ace is mediated by quorum sensing through the action of GelE, providing insight into the complicated world of Gram-positive pathogen adhesion and colonization.
机译：Ace，一种已知的毒力因子，是最早识别出与宿主细胞粘附和心内膜炎有关的粪肠球菌粘附基质分子（MSCRAMM）的微生物表面成分。粪肠球菌的Fsr群体感应系统是一种两组分信号转导系统，也已与粪肠球菌的毒力反复关联，部分原因是细胞外金属蛋白酶，明胶酶（GelE）的转录诱导。在这项研究中，我们发现Fsr途径的破坏显着增加了生长后期细胞表面Ace的水平。此外，我们观察到，除了 fsrB 突变体，其他被鉴定为缺乏GelE活性的菌株也表现出相似的表型。额外的实验证明了粪肠球菌表面的Ace依赖GelE裂解，证实GelE特异性地降低了Ace细胞表面展示。此外，Fsr系统或GelE表达的破坏显着提高了粪肠球菌粘附胶原的能力，这与粪肠球菌表面Ace含量较高相一致。这些结果表明，Ace的显示是通过GelE作用的群体感应来介导的，从而提供了对革兰氏阳性病原体粘附和定植的复杂世界的洞察力。

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