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首页> 外文期刊>Journal of bacteriology >Influence of Temperature on tRNA Modification in Archaea: Methanococcoides burtonii (Optimum Growth Temperature [Topt], 23°C) and Stetteria hydrogenophila (Topt, 95°C)
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Influence of Temperature on tRNA Modification in Archaea: Methanococcoides burtonii (Optimum Growth Temperature [Topt], 23°C) and Stetteria hydrogenophila (Topt, 95°C)

机译：温度对古细菌中tRNA修饰的影响：Burthanii甲烷菌（最佳生长温度[Topt]，23°C）和嗜水Stetteria嗜氢菌（Topt，95°C）

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摘要

We report the first study of tRNA modification in psychrotolerant archaea, specifically in the archaeon Methanococcoides burtonii grown at 4 and 23°C. For comparison, unfractionated tRNA from the archaeal hyperthermophile Stetteria hydrogenophila cultured at 93°C was examined. Analysis of modified nucleosides using liquid chromatography-electrospray ionization mass spectrometry revealed striking differences in levels and identities of tRNA modifications between the two organisms. Although the modification levels in M. burtonii tRNA are the lowest in any organism of which we are aware, it contains more than one residue per tRNA molecule of dihydrouridine, a molecule associated with maintenance of polynucleotide flexibility at low temperatures. No differences in either identities or levels of modifications, including dihydrouridine, as a function of culture temperature were observed, in contrast to selected tRNA modifications previously reported for archaeal hyperthermophiles. By contrast, S. hydrogenophila tRNA was found to contain a remarkable structural diversity of 31 modified nucleosides, including nine methylated guanosines, with eight different nucleoside species methylated at O-2′ of ribose, known to be an effective stabilizing motif in RNA. These results show that some aspects of tRNA modification in archaea are strongly associated with environmental temperature and support the thesis that posttranscriptional modification is a universal natural mechanism for control of RNA molecular structure that operates across a wide temperature range in archaea as well as bacteria.
机译：我们报道了在抗精神病的古细菌中，特别是在4°C和23°C下生长的古细菌Meemocococcoides burtonii中的tRNA修饰的第一个研究。为了比较，研究了在93℃下培养的古生嗜热菌嗜热链球菌的未分级tRNA。使用液相色谱-电喷雾电离质谱法对修饰的核苷进行分析后发现，两种生物体在tRNA修饰的水平和特性上存在显着差异。尽管在 M中的修改级别。在我们知道的所有生物中，burtonii tRNA最低，每个tRNA分子的二氢尿苷中含有一个以上的残基，该分子与在低温下维持多核苷酸的柔性有关。与先前报道过的古生嗜热菌的所选tRNA修饰相反，未观察到身份或修饰水平（包括二氢尿苷）随培养温度的变化。相比之下， S。嗜氢菌tRNA被发现具有31种修饰核苷的显着结构多样性，其中包括9种甲基化鸟苷，其中8种不同核苷在核糖的O-2'处甲基化，这被认为是RNA中的有效稳定基序。这些结果表明，古细菌中tRNA修饰的某些方面与环境温度密切相关，并支持以下论点：转录后修饰是控制RNA分子结构的通用自然机制，可在古细菌和细菌中在很宽的温度范围内运行。

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