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首页> 外文期刊>Journal of bacteriology >MorA Defines a New Class of Regulators Affecting Flagellar Development and Biofilm Formation in Diverse Pseudomonas Species
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MorA Defines a New Class of Regulators Affecting Flagellar Development and Biofilm Formation in Diverse Pseudomonas Species

机译:MorA定义了影响多种假单胞菌物种鞭毛发育和生物膜形成的新型调节剂。

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Assembly of bacterial flagella is developmentally important during both planktonic cell growth and biofilm formation. Flagellar biogenesis is complex, requiring coordinated expression of over 40 genes, and normally commences during the log-to-stationary transition phase. We describe here a novel membrane-localized regulator, MorA, that controls the timing of flagellar development and affects motility, chemotaxis, and biofilm formation in Pseudomonas putida. MorA is conserved among diverse Pseudomonas species, and homologues are present in all Pseudomonas genomes sequenced thus far. In P. putida, the absence of MorA derepresses flagellar development, which leads to constitutive formation of flagella in the mutant cells in all growth phases. In Pseudomonas aeruginosa, the absence of MorA led to a reduction in biofilm formation. However, unlike the motility of P. putida, the motility of the P. aeruginosa mutants was unaffected. Our data illustrate a novel developmentally regulated sensory and signaling pathway for several properties required for virulence and ecological fitness of Pseudomonas species.
机译:在浮游细胞生长和生物膜形成期间,细菌鞭毛的组装在发展上很重要。鞭毛生物发生很复杂,需要协调表达40多个基因,并且通常在从对数到平稳的过渡阶段开始。我们在这里描述了一种新型的膜局部调节剂MorA,它可以控制鞭毛发育的时间,并影响恶臭假单胞菌的运动性,趋化性和生物膜形成。 MorA在不同的

假单胞菌物种之间是保守的,到目前为止,所有

假单胞菌基因组中都存在同源物。在 P中。在恶臭中,缺乏MorA会抑制鞭毛发育,从而导致鞭毛在所有生长期的突变细胞中组成性地形成。在铜绿假单胞菌中,MorA的缺乏导致生物膜形成的减少。但是,与 P的活力不同。 putida P的运动能力。铜绿假单胞菌突变体不受影响。我们的数据说明了假单胞菌物种的毒力和生态适应性所需的几种特性的新型发育调控的感觉和信号传导途径。

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