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首页> 外文期刊>Journal of bacteriology >The Iron-Regulated iupABC Operon Is Required for Saprophytic Growth of the Intracellular Pathogen Rhodococcus equi at Low Iron Concentrations
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The Iron-Regulated iupABC Operon Is Required for Saprophytic Growth of the Intracellular Pathogen Rhodococcus equi at Low Iron Concentrations

机译：在低铁浓度下，胞内病原体红球菌的腐生生长需要铁调节的iupABC操纵子

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摘要

Rhodococcus equi is a facultative intracellular pathogen which proliferates rapidly in both manure-enriched soil and alveolar macrophages. Although both environments are characterized by extremely low concentrations of free iron, very little is known regarding the strategies employed by R. equi to thrive under these conditions. This paper reports the characterization of an R. equi transposome mutant that fails to grow at low iron concentrations. The transposome was shown to be inserted into iupA, the first gene of the iupABC operon encoding an ABC transport system highly similar to siderophore uptake systems. Disruption of the iupA gene also resulted in a failure of R. equi to utilize heme and hemoglobin as a source of iron. Introduction of the iupABC operon in trans restored the wild-type phenotype of the mutant strain. iupABC transcripts were 180-fold more abundant in R. equi grown in iron-depleted medium than in organisms grown in iron-replete medium. Proliferation of the iupABC mutant strain in macrophages was comparable to that of the wild-type strain. Furthermore, the iupABC mutant was not attenuated in mice, showing that the iupABC operon is not required for virulence.
机译： Rhodococcus equi 是一种兼性的细胞内病原体，在富含肥料的土壤和肺泡巨噬细胞中均迅速增殖。尽管两种环境都以极低的游离铁浓度为特征，但对于 R所采用的策略知之甚少。在这些条件下兴旺发达本文报告了 R的特征。在低铁浓度下无法生长的equi转座体突变体。已显示该转座子插入到 iupA 中，这是 iupABC 操纵子的第一个基因，其编码与铁载体吸收系统高度相似的ABC转运系统。 iupA 基因的破坏也导致 R的失败。可以利用血红素和血红蛋白作为铁源。在 trans 中引入 iupABC 操纵子可恢复突变菌株的野生型表型。 iupABC 转录本在 R中的含量高180倍。在贫铁培养基中生长的生物比在贫铁培养基中生长的生物高。 iupABC 突变菌株在巨噬细胞中的增殖与野生型菌株相当。此外， iupABC 突变体在小鼠中未减毒，表明 iupABC 操纵子不需要毒力。

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