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首页> 外文期刊>Journal of bacteriology >Synergistic Effects in Mixed Escherichia coli Biofilms: Conjugative Plasmid Transfer Drives Biofilm Expansion
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Synergistic Effects in Mixed Escherichia coli Biofilms: Conjugative Plasmid Transfer Drives Biofilm Expansion

机译：混合大肠杆菌生物膜中的协同效应：共轭质粒转移驱动生物膜扩展。

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Bacterial biofilms, often composed of multiple species and genetically distinct strains, develop under complex influences of cell-cell interactions. Although detailed knowledge about the mechanisms underlying formation of single-species laboratory biofilms has emerged, little is known about the pathways governing development of more complex heterogeneous communities. In this study, we established a laboratory model where biofilm-stimulating effects due to interactions between genetically diverse strains of Escherichia coli were monitored. Synergistic induction of biofilm formation resulting from the cocultivation of 403 undomesticated E. coli strains with a characterized E. coli K-12 strain was detected at a significant frequency. The survey suggests that different mechanisms underlie the observed stimulation, yet synergistic development of biofilm within the subset of E. coli isolates (n = 56) exhibiting the strongest effects was most often linked to conjugative transmission of natural plasmids carried by the E. coli isolates (70%). Thus, the capacity of an isolate to promote the biofilm through cocultivation was (i) transferable to the K-12 strain, (ii) was linked with the acquisition of conjugation genes present initially in the isolate, and (iii) was inhibited through the presence in the cocultured K-12 strain of a related conjugative plasmid, presumably due to surface exclusion functions. Synergistic effects of cocultivation of pairs of natural isolates were also observed, demonstrating that biofilm promotion in this system is not dependent on the laboratory strain and that the described model system could provide relevant insights on mechanisms of biofilm development in natural E. coli populations.
机译：通常由多种物种和遗传上不同的菌株组成的细菌生物膜在细胞-细胞相互作用的复杂影响下形成。尽管已经出现了有关单一物种实验室生物膜形成机制的详细知识，但对控制更复杂的异质群落发展的途径知之甚少。在这项研究中，我们建立了一个实验室模型，其中监测了由于大肠杆菌的遗传多样性菌株之间相互作用而引起的生物膜刺激作用。 403未内陷的 E的共培养导致生物膜形成的协同诱导。具有特征性 E的大肠杆菌菌株。大肠杆菌K-12菌株的检出频率很高。这项调查表明，观察到的刺激是不同的机制，但 E子集内生物膜的协同发展。表现出最强作用的大肠埃希菌分离株（ n = 56）通常与 E携带的天然质粒的结合传递有关。大肠埃希菌分离株（占70％）。因此，分离物通过共培养促进生物膜的能力是（i）可转移至K-12菌株，（ii）与分离物中最初存在的结合基因的获取有关，并且（iii）通过分离得到抑制。可能是由于表面排斥功能，在共培养的K-12菌株中存在相关的结合质粒。还观察到了一对自然分离株共培养的协同效应，表明该系统中生物膜的促进不依赖于实验室菌株，并且所描述的模型系统可以提供有关天然 E中生物膜发展机制的相关见解。大肠菌群。

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