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首页> 外文期刊>Journal of bacteriology >Cation-Induced Transcriptional Regulation of the dlt Operon of Staphylococcus aureus
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Cation-Induced Transcriptional Regulation of the dlt Operon of Staphylococcus aureus

机译：阳离子诱导的金黄色葡萄球菌dlt操纵子的转录调控。

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Lipoteichoic and wall teichoic acids (TA) are highly anionic cell envelope-associated polymers containing repeating polyglycerol/ribitol phosphate moieties. Substitution of TA with d-alanine is important for modulation of many cell envelope-dependent processes, such as activity of autolytic enzymes, binding of divalent cations, and susceptibility to innate host defenses. d-Alanylation of TA is diminished when bacteria are grown in medium containing increased NaCl concentrations, but the effects of increased salt concentration on expression of the dlt operon encoding proteins mediating d-alanylation of TA are unknown. We demonstrate that Staphylococcus aureus transcriptionally represses dlt expression in response to high concentrations of Na+ and moderate concentrations of Mg2+ and Ca2+ but not sucrose. Changes in dlt mRNA are induced within 15 min and sustained for several generations of growth. Mg2+-induced dlt repression depends on the ArlSR two-component system. Northern blotting, reverse transcription-PCR, and SMART-RACE analyses suggest that the dlt transcript begins 250 bp upstream of the dltA start codon and includes an open reading frame immediately upstream of dltA. Chloramphenicol transacetylase transcriptional fusions indicate that a region encompassing the 171 to 325 bp upstream of dltA is required for expression and Mg2+-induced repression of the dlt operon in S. aureus.
机译：脂磷壁酸和壁磷壁酸（TA）是高度阴离子细胞包膜相关的聚合物，包含重复的聚甘油/核糖醇磷酸基团。用d-丙氨酸替代TA对调节许多细胞包膜依赖性过程非常重要，例如自溶酶的活性，二价阳离子的结合以及对先天宿主防御的敏感性。当细菌在含有增加的NaCl浓度的培养基中生长时，TA的d-丙氨酰化作用减弱，但是盐浓度增加对介导TA的d-丙氨化的 dlt 操纵子编码蛋白表达的影响尚不清楚。我们证明了金黄色葡萄球菌转录抑制高浓度的Na + 和中等浓度的Mg 2 + 的表达sup>和Ca 2 + 而不是蔗糖。 dlt mRNA的变化在15分钟内被诱导并持续了几代的生长。 Mg 2 + 诱导的 dlt 抑制取决于ArlSR两组分系统。 Northern印迹，逆转录PCR和SMART-RACE分析表明， dlt 转录本在 dltA 起始密码子上游250 bp处开始，并包括一个紧邻其上游的开放阅读框。 dltA 。氯霉素转乙酰基酶的转录融合表明，表达 dltA 上游需要一个包含171至325 bp的区域，而Mg 2 + 诱导的 dlt 的阻遏作用 S中的em>操纵子。金黄色。

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