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首页> 外文期刊>Journal of bacteriology >Short-Tailed Stx Phages Exploit the Conserved YaeT Protein To Disseminate Shiga Toxin Genes among Enterobacteria
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Short-Tailed Stx Phages Exploit the Conserved YaeT Protein To Disseminate Shiga Toxin Genes among Enterobacteria

机译：短尾Stx噬菌体利用保守的YaeT蛋白在肠细菌中传播志贺毒素基因

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Infection of Escherichia coli by Shiga toxin-encoding bacteriophages (Stx phages) was the pivotal event in the evolution of the deadly Shiga toxin-encoding E. coli (STEC), of which serotype O157:H7 is the most notorious. The number of different bacterial species and strains reported to produce Shiga toxin is now more than 500, since the first reported STEC infection outbreak in 1982. Clearly, Stx phages are spreading rapidly, but the underlying mechanism for this dissemination has not been explained. Here we show that an essential and highly conserved gene product, YaeT, which has an essential role in the insertion of proteins in the gram-negative bacterial outer membrane, is the surface molecule recognized by the majority (ca. 70%) of Stx phages via conserved tail spike proteins associated with a short-tailed morphology. The yaeT gene was initially identified through complementation, and its role was confirmed in phage binding assays with and without anti-YaeT antiserum. Heterologous cloning of E. coli yaeT to enable Stx phage adsorption to Erwinia carotovora and the phage adsorption patterns of bacterial species possessing natural yaeT variants further supported this conclusion. The use of an essential and highly conserved protein by the majority of Stx phages is a strategy that has enabled and promoted the rapid spread of shigatoxigenic potential throughout multiple E. coli serogroups and related bacterial species. Infection of commensal bacteria in the mammalian gut has been shown to amplify Shiga toxin production in vivo, and the data from this study provide a platform for the development of a therapeutic strategy to limit this YaeT-mediated infection of the commensal flora.
机译：志贺毒素编码噬菌体（Stx噬菌体）感染大肠杆菌是致命志贺毒素编码 E进化过程中的关键事件。大肠杆菌（STEC），其中以O157：H7血清型最为臭名昭著。自1982年首次报道STEC感染暴发以来，据报道可产生志贺毒素的不同细菌和菌株的数量现已超过500种。显然，Stx噬菌体正在迅速传播，但尚未阐明这种传播的潜在机制。在这里，我们显示了一种重要且高度保守的基因产物YaeT，其在革兰氏阴性细菌外膜中插入蛋白质中具有重要作用，是大多数（约70％）Stx噬菌体识别的表面分子。通过与短尾形态相关的保守的尾穗蛋白。 yaeT 基因最初是通过互补来鉴定的，其作用在有和没有抗YaeT抗血清的噬菌体结合测定中得到了证实。 E的异源克隆。大肠杆菌yaeT 能够使Stx噬菌体吸附到 cartovora ，而具有天然 yaeT 变体的细菌物种的噬菌体吸附模式进一步支持了这一结论。大多数Stx噬菌体使用必不可少且高度保守的蛋白质是一种策略，已启用并促进了致毒素毒素潜力在多个Eem中的快速传播。大肠菌群及相关细菌种类。哺乳动物肠道中共生细菌的感染已显示可在体内扩增志贺毒素的产生，这项研究的数据为开发治疗策略提供了平台，以限制这种YaeT介导的共生菌感染。

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